无患子总皂苷体外抑制酪氨酸酶活性的研究  

Research on the Inhibition of Tyrosinase Activity in vitro by Total Saponins from Sapindus

作　　者：陈雷[1] 赵春琳[1] 周生学[1] 

机构地区：[1]吉林农业科技学院中药学院,吉林吉林130011

出　　处：《安徽农业科学》2015年第21期56-58,共3页Journal of Anhui Agricultural Sciences

基　　金：吉林农业科技学院大学生科技创新科研项目(2014052)

摘　　要：[目的]研究无患子总皂苷体外对酪氨酸酶的抑制率。[方法]超声波提取法,溶剂用水、30%乙醇和60%乙醇对无患子总皂苷提取,用大孔树脂(D101)富集水、30%乙醇和70%乙醇部分,紫外分光光度计法,测量酪氨酸酶抑制率。[结果]超声波提取得率中,水提得率(18.56%)最高,60%乙醇提取得率为14.46%,而30%乙醇提取得率为17.85%,接近水提取得率;大孔树脂富集后70%乙醇洗脱部分抑制率高于30%乙醇部分和水部分;与熊果酸比较,70%乙醇洗脱部分、30%乙醇洗脱部分和总皂苷对酪氨酸酶均具有明显的抑制作用。[结论]无患子总皂苷及30%乙醇和70%乙醇大孔树脂洗脱部位对酪氨酸酶抑制率较高,可以作为优良的美白护肤品天然添加剂,应用于生产中。[ Objective ] The research aimed to study the inhibition rate of tyrosinase in vitro by total saponins from Sapindus. [ Method ] Total saponins were extracted from Sapindus by Ultrasonic with water, 30% ethanol and 60% ethanol. Each part of the water, 30% ethanol and 70% ethanol was collected through macroporous resin （D101） column. Tyrosinase inhibition rate was measured by ultraviolet spectrophotometer. [ Result] Total saponius extract yield （ 18.56% ） by water was higher than 60% ethanol （ 14.46% ） and 30% ethanol （ 17.85% ） , and 30% ethanol content close to water extraction yield. The tyrosinase inhibitory activity with 70% ethanol eluent was higher than 30% ethanol eluent by different parts of Macroporous resin elution of Sapindus. Compared with ursolic acid, 70% ethanol eluent, 30% ethanol eluent and total saponins were significantly tyrosiuase inhibited. [ Conclusion] The inhibition rate of total saponins of Sapindus and 30% ethanol and 70% ethanol of macroporeus rosin elution fractions on tyrosinase were higher, which can be used as an excellent natural skin care products whitening additive, used in production.

关 键 词：无患子 总皂苷 酪氨酸酶 体外 抑制率 

分 类 号：S567[农业科学—中草药栽培]