黄酮类化合物GL-V9对人胃低分化黏液腺癌细胞株的凋亡作用及其机制  

Effects and mechanism of programmed cell death of human gastric cancer cell under the treatment of flavonoid compound GL-V9

作　　者：李国君[1,2] 赵凯[1,2] 姚彧远 申乐[1,2] 郭青龙[1,2] 

机构地区：[1]中国药科大学江苏省肿瘤发生与干预重点实验室,南京210009 [2]中国药科大学天然药物活性组分与药效国家重点实验室,南京210009

出　　处：《中国药科大学学报》2015年第4期464-468,共5页Journal of China Pharmaceutical University

基　　金：国家"重大新药创制"科技重大专项资助项目(No.2013ZX09103-001-007)~~

摘　　要：探讨黄酮类化合物GL-V9对人体低分化胃黏液腺癌细胞系MGC-803和BGC-823的凋亡作用及其机制。采用MTT法观察不同浓度的GL-V9作用于两种胃癌细胞后对其细胞活力的影响。采用Annexin V-FITC/PI双染法、DAPI染色法观察GL-V9对MGC-803细胞株凋亡率和细胞核形态的影响。采用免疫印迹法观察GL-V9对MGC-803细胞株中主要凋亡蛋白caspase-9,caspase-3的影响。应用钙离子荧光探针Fluo-3 AM检测GL-V9对MGC-803细胞株内钙离子浓度变化的影响。实验结果表明,GL-V9可以抑制胃癌细胞株MGC-803,BGC-823细胞活性,改变MGC-803细胞核形态,激活caspase-9,caspase-3蛋白,诱导细胞凋亡。同时,GL-V9可以显著上调MGC-803细胞中钙离子水平,这可能与GL-V9作用下的胃癌细胞株中线粒体凋亡通路的激活相关。To investigate the apoptotic effect of flavonoid compound GL-V9 on human gastric cancer cells and its potential mechanism, MGC-803 and BGC-823 cells were treated with GL-V9. MT-F assay was performed to assess the growth inhibition effects on MGC-803 and BGC-823 cells under different concentrations of GL-V9. Annexin V-FITC/PI staining assay was employed to observe the apoptotic rate of GL-V9 cells with the treatment of GL- V9. DAPI staining was performed to observe the nuclear morphological changes using fluorescence microsco- py. Activation of caspase-9 and caspase-3 was analyzed by Western blotting. Ca2~ concentration in gastric cancer cells was detected by Fluo-3 AM staining assay. Results showed that GL-V9 could inhibiteell viability, change the nuclear morphologyl, activate caspase-9 and caspase-3 and induce the apoptosis in gastric cancer cells. The mech- anism of the induction of apoptosis in MGC-803 cells under the treatment of GL-V9 may aetivate the Ca2＋ associ- ated mitochondrial apoptosis pathway.

关 键 词：黄酮类化合物 GL—V9 胃腺癌 MGC-803细胞 凋亡 钙离子 

分 类 号：R285[医药卫生—中药学]