乌贼墨黑色素对小鼠脏器系数的影响及排铅效果的研究  

The Effect of Sepia Melanin on Organ Coefficient and Excreting Lead in Mice

作　　者：吕玲[1] 刘漫[1] 李和生[1] 

机构地区：[1]宁波大学海洋学院食品资源利用研究室,宁波315211

出　　处：《天然产物研究与开发》2015年第7期1162-1165,1204,共5页Natural Product Research and Development

基　　金：宁波市自然科学基金(2013A610156);"水产"浙江省重中之重学科开放基金(xkzsc1426);浙江省优先主题计划(2009C03017-3)

摘　　要：本文研究了乌贼墨黑色素对小鼠脏器系数的影响以及对铅中毒小鼠的排铅效果,采用腹腔注射醋酸铅溶液建立铅中毒小鼠实验模型,对铅中毒小鼠进行乌贼墨黑色素灌胃治疗,24 h后取眼球血,断颈处死并取肝脏及脑组织,用电感耦合等电子体发射光谱(ICP-AES)测定小鼠血液、肝脏以及脑组织中的铅含量。结果表明,乌贼墨黑色素对小鼠肝脏和脑的脏器系数无显著影响;小鼠染铅30 min后,用乌贼墨黑色素灌胃1次进行治疗,治疗组小鼠的肝、血液中的铅含量与铅模型组相比差异极显著(P<0.01),脑铅下降不明显。小鼠染铅24 h后,用乌贼墨黑色素灌胃1次进行治疗,治疗组小鼠的肝、血液中的铅含量与铅模型组相比差异极显著(P<0.01),脑铅下降差异显著(P<0.05)。乌贼墨黑色素对24 h内铅中毒小鼠有显著的排铅效果。To study the effect of sepia melanin on mice’s organ coefficient and lead excretion in mice suffering lead poisoning,the experimental model of lead-poisoning mice were established via intraperitoneal injection of lead acetic solution.Gavage treatment of sepia melanin was given to those poisoning mice and the blood was collected by picking the eyeballs of mouse after 24 h.Then,mice were killed by breaking necks to acquire liver and brain tissue.The lead contents in blood,liver and brain were determined by inductively coupled plasma atomic emission spectrometer （ICP-AES）.The results showed that sepia melanin had no significant effect on organ coefficient of mice.After 30 min poisoning by lead,mice were given gavage treatment by sepia melanin.The contents of lead in blood and liver in the treated group were lower than those in model group （P〈0.01）.After 24 h poisoning by lead,mice were given gavage treatment by sepia melanin.The lead contents in blood and liver in the treated group were lower than those in model group （P〈0.01） and the lead content in brain was also decreased （P〈0.05）.This experiment indicated sepia melanin had significant effects on removing lead of mice within 24 h lead poisoning.

关 键 词：乌贼墨黑色素 铅离子 排铅 脏器系数 

分 类 号：R595.2[医药卫生—内科学]