基于近红外光谱法的枳实含量测定  

Determination of Citrus aurantium Based on Near-infrared Spectroscopy

作　　者：雷敬卫[1] 樊明月[1,2] 白雁[1] 谢彩侠[1] 张迪文 

机构地区：[1]河南中医学院,郑州450046 [2]新乡市第二人民医院,新乡453002

出　　处：《天然产物研究与开发》2015年第7期1199-1204,共6页Natural Product Research and Development

基　　金：河南中医学院科技创新人才项目(2014XCXRC03);河南省中药开发工程技术研究中心项目(142102313109);河南省教育厅科学技术研究重点项目(14B360007)

摘　　要：本文应用近红外漫反射光谱法快速测定枳实中辛弗林与醇浸出物的含量。采用高效液相色谱法测定辛弗林的含量,以热浸法测定醇浸出物的含量,运用近红外光谱技术结合化学计量学方法建立枳实中辛弗林与醇浸出物的定量分析模型。并用未知样品验证该模型。结果表明辛弗林与醇浸出物的定量分析模型的内部交叉验证相关系数(R2)分别为0.98052、0.98489,校正均方差(RMSEC)分别为0.00915、0.153,预测均方差(RMSEP)分别为0.0119、0.188,验证集的预测相关系数(r)分别为0.9106、0.9484。该方法简便、快速、准确,可用于枳实中辛弗林与醇浸出物含量的快速测定。This study determined the content of synephrine and ethanol-extract in Citrus aurantium using near-infrared spectroscopy.The determination results were reconfirmed with HPLC analysis and hot dip assay.The near-infrared spectroscopy was combined with chemometrics to establish the quantitative model of synephrine and ethanol-extract in C.aurantium. The correlation coefficients （R^2） of synephrine and ethanol-extract were 0.98052 and 0.98489,respectively.The root mean-square error of calibration （RMSEC） were 0.00915 and 0.153,respectively.The root-mean-square error of predication （RMSEP） were 0.0119 and 0.188, respectively. The validation coefficients （r） were 0.9106 and 0.9484,respectively.The developed method was convenient,fast and accurate.It was feasible to determine the content of synephrine and ethanol-extract in C.aurantium.

关 键 词：近红外光谱技术 枳实 测定 

分 类 号：O657.33[理学—分析化学]