补肾固筋方对SD大鼠膝骨关节炎IL-1、iNOS分泌的抑制作用  

Inhibitory Effect of Bu-Shen Gu-Jin Prescription on Interleukin-1 and Inducible Nitric Oxide Synthase in SD Rat Knee Osteoarthritis

作　　者：张冲[1] 渠玲玲 王恒树[3] 张春利[3] 李双成[2] 黄芸[2] 吴中秋[4] 

机构地区：[1]河北省中医院骨三科,石家庄050000 [2]河北医科大学,石家庄050011 [3]河北医科大学第二医院,石家庄050011 [4]河北中医学院,石家庄050017

出　　处：《天然产物研究与开发》2015年第7期1266-1269,1316,共5页Natural Product Research and Development

基　　金：河北省中医药类科研技术指导性课题(2014172)

摘　　要：探讨中药补肾固筋方对SD大鼠膝骨关节炎中IL-1、TNF-α的抑制作用。44只SD大鼠随机分为正常组、予生理盐水的模型组、美洛昔康组和补肾固筋方组,除正常组外均采用右后膝改良Hulth法造模,连续干预12周,取血清及股骨内髁关节软骨、滑液及软骨下骨,ELISA法检测IL-1(白细胞介素-1)、i NOS(诱导型一氧化氮合酶)含量。结果发现美洛昔康组关节间隙狭窄、关节面粗糙,骨赘明显重于补肾固筋方组;补肾固筋方组关节间隙稍窄伴轻微骨赘。模型组IL-1、i NOS含量较正常组升高(P<0.01);补肾固筋方组和美洛昔康组含量比模型组降低(P<0.01)且两组之间差异无统计学意义(P>0.05)。提示补肾固筋方通过有效抑制IL-1、i NOS的分泌促进骨关节炎软骨修复。The aim of this study was to explore the inhibitory effect of traditional Chinese medicine Bu-Shen Gu-Jin prescription （BSGJP） on the secretion of interleukin-1 （IL-1） and inducible nitric oxide synthase （iNOS） in SD rat knee osteoarthritis.A total of 44 SD rats were randomly divided into normal group,model group,meloxicam model group and BSGJFP group,n=11 for each group.The rats were modeled of improved Hulth method on the right hind limb knee cartilage except for the normal group.After 12 weeks’ treatment,the expressions of IL-1,iNOS in serum,synovial fluid and osteoarthritis of rats were detected and compared by means of enzymelinked immusorbent assay.The results showed that the articular surface of meloxicam group was rough and joint space of meloxicam group was narrow.The osteophyte of meloxicam group was significantly heavier than that of BSGJP group.The expression of IL-1 and iNOS of model group was significantly higher （P〈0.01） than that of normal group,while the levels of IL-1 and iNOS in BSGJP group and meloxicam group were significant lower than those of other groups （P〈0.01）. No significant difference （P〉0.05） of IL-1 and iNOS levels was detected between these two groups.The results demonstrated that BSGJP can play a role in promoting cartilage repair by effectively suppress the secretion of IL-1 and the expression of iNOS.Our study provided a preliminary experimental basis for using BSGJP as a clinical drug.

关 键 词：补肾固筋方 美洛昔康 膝骨关节炎 软骨细胞 白细胞介素-1 诱导型一氧化氮合酶 

分 类 号：Q939.9[生物学—微生物学]