黄芩等三味具有抑菌活性中药的最佳提取工艺研究  

Optimization of Extraction of 3 Traditional Chinese Medicines with Antibacterial Activity

作　　者：彭练慈[1] 殷中琼[1] 康帅[1] 曲径[1] 刘明辉[1] 陈萍[1] 

机构地区：[1]四川农业大学动物医学院,雅安625014

出　　处：《天然产物研究与开发》2015年第7期1273-1279,1218,共8页Natural Product Research and Development

基　　金：四川省青年科技创新研究团队(2013TD0015);"十二五"农村领域国家科技计划项目(2011BAD34B03)

摘　　要：以浸膏得率、最低抑菌浓度(MIC)值和最低杀菌浓度(MBC)为指标,采用正交设计试验,对黄芩等三味中药有效物质提取工艺进行研究。优选出黄芩等三味中药的最佳提取工艺为:黄连粗粉80%乙醇浓度(8倍量),加热回流提取2次,每次1.5 h;黄芩粗粉55%乙醇浓度(10倍量),95℃加热回流提取3次,每次2 h;虎杖粗粉60%乙醇浓度(5倍量),95℃加热回流提取3次,每次2 h。在最佳提取工艺研究下,黄连、黄芩和虎杖三种提取物浸膏对无乳链球菌的MIC值分别为26.04、5.20、15.60 mg/m L,MBC值分别为26.04、6.50、31.25 mg/m L。此提取工艺操作简便,稳定可行,值得借鉴。In order to establish the optimal methods for the extraction of 3 traditional Chinese medicines,the extractive yield,minimum inhibition concentration （MIC） and minimal bactericidal concentration （MBC） against Streptococcus agalactiae were used as comprehensive evaluation indexes.L9（34） orthogonal design was applied to select the optimal extraction conditions of 3 traditional Chinese medicines.The results showed that the optimal extraction conditions of 3 traditional Chinese medicines were as follows：Coptidis Rhizoma was added to 8 times of 80% ethanol,which were refluxed and extracted for 2 times with each time of 1.5 h;Radix Scutellariae were extracted with 10 times of 55% ethanol at 95 ℃,and repeated for 3 times,2 h for each time; Polygonum cuspidatum extracted 3 times at 95 ℃ with 5 times of 60% ethanol,2 h for each time.Under optimal conditions,the MICs of the extracts obtained from Coptidis Rhizoma,Radix Scutellariae and Polygonum cuspidatum against S.agalactiae were 26.04,5.20 mg/mL and 15.60 mg/mL,respectively and the MBCs were 26.04,6.50,31.25 mg/mL respectively.The developed extraction method was simple,rapid,accurate,reproducible and stable.

关 键 词：中药 提取工艺 正交设计 浸膏得率 

分 类 号：S852.6[农业科学—基础兽医学]