HPLC-Q-TOF MS鉴定条叶榕根茎乙酸乙酯提取物中的主要化学成分  

Identification and Characterization of Major Chemical Compounds in the Ethyl Acetate Extract fromFicus pandurata Hance Aerial Roots by HPLC-Q-TOF MS

作　　者：张小平[1] 蒋可志[2] 吕惠卿[3] 聂晶[1] 李祖光[1] 

机构地区：[1]浙江工业大学化学工程学院,浙江杭州310014 [2]杭州师范大学有机硅重点实验室,浙江杭州311121 [3]浙江中医药大学药物科学院,浙江杭州310053

出　　处：《质谱学报》2015年第4期310-320,共11页Journal of Chinese Mass Spectrometry Society

基　　金：浙江省中青年学科带头人学术攀登项目(Pd2013016);国家自然科学基金项目(21205025);浙江省科技厅项目(2015C32006);2014年杭州市钱江特聘专家和农业部农药残留检测重点实验室项目资助

摘　　要：采用高效液相色谱-四极杆飞行时间质谱(HPLC-Q-TOF MS)技术对条叶榕根茎乙酸乙酯提取物中的多种活性化学组分进行了分析鉴定。采用正离子与负离子两种扫描方式,依据高分辨质谱提供的准分子离子峰和碎片离子的精确分子质量信息,确证了有关物质及其特征碎片离子的分子组成。再结合标准品对照与相关文献数据支持,最终鉴定出条叶榕根茎乙酸乙酯提取物中的34种化学成分,其中包括12种多酚、8种香豆素、9种黄酮、4种脂肪酸、1种蒽醌。结果表明,采用HPLC-Q-TOF MS技术可提高中药化学成分的分析效率,有利于新化合物的发现与鉴别。The major chemical compounds in the ethyl acetate extract fromFicus pandurata Hance aerial roots were analyzed using high performance liquid chromatographycoupled with quadrupole time-of-flight mass spectrometry（HPLC-Q-TOF MS）.The ESI-MS analysis was operated at the mass range of m/z100-800 under both the positive and the negative ionization modes,respectively.A total of 34 compounds,such as 12 phenolics,8coumarins,9flavonoids,4fatty acids,and 1anthraquinone,which are characterized in the ethyl acetate extract,based on the accurate mass of molecular and product ions provided by Q-TOF MS,comparison with standard substances and references.It is an effective method to provide chemical information concerning the constituents in herbal medicines,which makes component analysis more convinced.

关 键 词：高效液相色谱-四极杆飞行时间质谱(HPLC-Q-TOF MS) 条叶榕 化学组分 碎片离子 

分 类 号：O657.63[理学—分析化学]