辣椒花药培养再生植株的倍性鉴定  

Ploidy Identification in Regenerated Pepper Plants from Anther Culture

作　　者：付文婷[1] 何建文[1] 杨红[1] 邢丹[1] 张爱民[1] 苏丹[1] 韩世玉[1] 

机构地区：[1]贵州省辣椒研究所,贵州贵阳550006

出　　处：《贵州农业科学》2015年第6期18-21,共4页Guizhou Agricultural Sciences

基　　金：贵州省科技计划项目"辣椒;蚕桑种质资源开发利用研究创新能力建设"[黔科合院所创新(2012)4003];贵州省农业科学院研究生创新基金项目"贵州辣椒地方品种花药培养研究"[黔农科合创新基金(2011004)]

摘　　要：为指导辣椒单倍体育种技术在实践中的应用,对辣椒8024花药培养再生植株的染色体倍性进行鉴定,以DNA流式细胞仪测定法的鉴定结果为依据,研究利用染色体压片计数法和花药培养再生植株形态及坐果情况鉴定辣椒花药培养再生植株倍性的可靠性。结果表明,花药培养再生植株是倍性水平不同的混倍群体,但各倍性植株所占的比例不同,DNA流式细胞仪测定法和染色体计数法的吻合度达93%,与坐果鉴定结果一致。鉴定结果可直接决定其结籽情况。To guide the application of haploid breeding technique in practice,the ploidy was identified in population of anther culture derived pepper 8024 plants.Based on the results of DNA flow cytometric（FCM）analytical technique,the authors studied the reliability of deriving pepper ploidy by using chromosome counting,fruit setting and morphology.Results：Regenerated populations from anther culture are usually mixed ploidy,the proportion of different ploidy levels are different.The results of which determine the seed setting status directly.The coincidence between FCM and chromosome counting is high up to 93%,which is in accordance with fruit setting results.

关 键 词：辣椒 花药培养 再生植株 流式细胞仪 染色体计数 倍性鉴定 

分 类 号：S641.3[农业科学—蔬菜学]