猪圆环病毒2型贵州株ORF1基因的生物信息学分析  

Bioinformatics Analysis of ORF1 Gene of Porcine Circovirus Type 2 in Guizhou

作　　者：杨利勇[1,2] 李春凤 周继勇[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310058 [2]贵州福斯特生物科技有限公司,贵州贵阳550014

出　　处：《贵州农业科学》2015年第6期123-127,共5页Guizhou Agricultural Sciences

基　　金：贵州省农业委员会项目"贵州省猪圆环病毒病流行病学调查研究"(2011-12-02)

摘　　要：为了解猪圆环病毒2型贵州株（PCV2-GZ）ORF1基因的生物学特性,针对ORF1基因设计1对特异性引物,取PCV2阳性病料DNA为模板进行PCR扩增,克隆测序后进行生物信息学分析。结果表明：PCV2-GZ ORF1基因序列与GenBank登录的14株PCV2（06-06274,CC1,HUB-5,HUN-11,JX-1,LN-3,P710-1,PCV2HERD D,SC-10,SVP-321,SX-1,VOS 2689006,ZJ-38,N1）相应序列核苷酸的同源性为96.8%～99.7%,与国内HUB-5和ZJ-38的同源性最高,均为99.7%;PCV2-GZ编码的Rep蛋白与P710-1、HUB-5、SC-10、ZJ-38株的氨基酸序列同源性较高,均为99.4%。PCV2-GZ Rep蛋白可形成优势抗原表位,且不存在跨膜结构和信号肽,推测可进行PCV2 ORF1基因的全基因表达。In order to analysis bioinformation of ORF1 gene of PCV2 in Guizhou,apair of primers aiming to ORF1 gene had been designed PCR amplification of PCV2ORF1 from the tissue was subcloned into vector pMD-18 T,which was screened by sequencing.Results：Comparing cloned PCV2ORF1（PCV2-GZ）with other 14 ORF1 sequences from GenBank（06-06274,CC1,HUB-5,HUN-11,JX-1,IN-3,P710-1,PCV2 HERD D,SC-10,SVP-321,SX-1,VOS 268900,ZJ-38,N1）,resulting in a high similarity between96.8%～99.7% as a whole,99.7%（HUB-5and ZJ-38）.The animo acid sequences of protein Rep also has a high similarity between P710-1,HUB-5,SC-10,ZJ-38 and PCV2-GZ with 99.4% as a whole.There＇re dominant epitopes in the Rep of PCV2-GZ without any signal peptide and transmembrane domain,so there＇s a possibility that express the full length of PCV2 ORF1.

关 键 词：猪圆环病毒2型 ORF1基因 生物信息学 贵州 

分 类 号：S852.65[农业科学—基础兽医学]