海藻酸钠荧光多孔支架的制备  

Preparation of sodium alginate fluorescent porous scaffolds

作　　者：罗桂林[1] 白家繁 汪建新[1] 张俊良 陈太军[1] 闫浩然[1] 冯波[1] 翁杰[1] 

机构地区：[1]西南交通大学材料科学与工程学院西南交通大学材料先进技术教育部重点实验室,四川成都610031 [2]黎明化工研究设计院有限责任公司,河南洛阳471000

出　　处：《化学推进剂与高分子材料》2015年第4期60-67,70,共9页Chemical Propellants & Polymeric Materials

基　　金：973国家重大科学研究计划(编号2012CB933601);国家自然基金(No.51072167和No.31370966)

摘　　要：采用乳化剂交联法,以海藻酸钠(SA)为原料,Ca Cl2为物理交联剂,制备了海藻酸钠多孔支架;然后分别以0.2 mol/L Zn(Ac)2、Zn Cl2和Zn(NO3)2为交联剂,在海藻酸钠溶液质量浓度为15 g/L,温度为8℃的条件下,制备了竖直贯通的多孔支架;冷冻干燥后,将支架浸泡在KOH/甲醇/无水乙醇混合溶液中(Zn O量子点原位合成法),制备了荧光竖直定向多孔支架。研究了海藻酸钠的质量浓度(5 g/L、10 g/L、15 g/L)及不同干燥方法(冷冻干燥和乙醇逐级脱水法)对制备海藻酸钠多孔支架的影响;将成纤维细胞与海藻酸钠多孔支架共培养,考察海藻酸钠多孔支架的生物相容性,采用Cy3(Cy–N–羟基琥珀酰亚胺酯)与DAPI(4,6–二脒基–2–苯基吲哚)双荧光染色法观察细胞生长情况及采用MTT(3–(4,5–二甲基噻唑–2)–2,5–二苯基四氢唑溴盐)法定量检测了海藻酸钠多孔支架的细胞毒性;对荧光多孔支架的形貌和荧光性能进行了表征。The sodium alginate porous scaffolds were prepared by the emulsifier cross-linking method using sodium alginate （SA） as raw material and CaC12 as physical cross-linking agent. The vertical through porous scaffolds were prepared by using 0.2 mol/L Zn（Ac）2, ZnCl2 and Zn（NO3）2 separately as the cross-linking agents when the temperature is 8 ℃, the mass concentration of the sodium alginate solution is 15 g/L. After freeze-drying, the scaffolds were immersed in the KOH/methanol/absolute ethanol mixed solution （ZnO quantum dot in-situ synthesis method）, and the fluorescence vertical orientation porous scaffolds were prepared. The influence of sodium alginate mass concentration （5 g/L, 10 g/L, 15 g/L） and different drying methods （freeze-drying method and step-dehydrating method with ethanol） on preparation of the sodium alginate porous scaffolds was investigated. The fibroblast cells and sodium alginate porous scaffolds were co-cultured, the bio-compatibility of the sodium alginate porous scaffolds was investigated. The cell growth was observed by using Cy3 （Cy-N-hydroxysuccinimide ester） and DAPI （4,6-diamidino- 2-phenylindole） double fluorescence staining method, and the cell cytotoxicity of the sodium alginate porous scaffolds was quantitatively detected by using MTT （3-（4,5-dimethylthiazole-2）-2,5-diphenyltetrahydro azole bromide salt） method. The morphology and fluorescence performance of the fluorescence porous scaffolds were characterized.

关 键 词：海藻酸钠 ZnO量子点 荧光多孔支架 生物相容性 

分 类 号：TQ314.1[化学工程—高聚物工业] R318.08[医药卫生—生物医学工程]