急性羰基镍中毒大鼠心脾肾组织SOD活力的动态观察  

Observation on SOD activity in heart,spleen and kidney of rats poisoned acute nickel carbonyl

作　　者：王龙[1] 王秋英 王宁[3] 吴喜江 马国煜 尚慧 宣小强 任秀芳 程宁[3] 

机构地区：[1]兰州大学公共卫生学院,甘肃兰州730000 [2]金川公司职工医院,甘肃金昌737103 [3]兰州大学甘肃省新药临床前研究重点实验室,甘肃兰州730000

出　　处：《工业卫生与职业病》2015年第4期280-283,共4页Industrial Health and Occupational Diseases

基　　金：金川集团公司与兰州大学产学研合作项目(金科综2009-12)

摘　　要：目的通过对羰基镍染毒大鼠心、脾、肾组织中超氧化物歧化酶(SOD)活力的动态观察,并比较不同脏器SOD活力变化的差异,探讨羰基镍的急性毒效应。方法健康SD大鼠静态吸入染毒,羰基镍低、中、高染毒浓度分别为20、135和250mg/m3,以氯气250mg/m3染毒为阳性对照组,均染毒30min,并设空白对照组。染毒后第1、2、3、7天取材,应用黄嘌呤氧化酶测定法分别测定各脏器中SOD活力。结果低、中浓度羰基镍染毒组心、脾、肾组织中SOD活力低于空白对照组,但差异无统计学意义(P>0.05),高浓度羰基镍组明显抑制SOD活力(P<0.05)。抑制作用以染毒后第3天最重(P<0.05),第7天时渐恢复,与阳性对照组比较,差异无统计学意义(P>0.05)。氯气染毒组心、脾组织中SOD活力抑制程度高于羰基镍染毒组,但高浓度羰基镍染毒组肾组织中SOD活力低于空白对照组。结论吸入羰基镍可致心、脾、肾组织中SOD活力不同程度的下降,诱发组织氧化损伤。Objective To investigate and compare the SOD activity in heart, spleen and kidney of rats poisoned by acute nickel carbonyl, and discuss the mechanism of its acute toxicity. Methods Healthy SD were exposed to nickel carbonyl in different concentrations （20,135 and 250 mg/m3 respectively） for 30 rain. Set SD rats exposed to 250 mg/m3 （30 min）chlorine as the positive control group, and healthy SD rats as the negative control group. Samples in different sites respectively were gotten after exposed 1, 3, 5 and 7 days. Then their SOD activities were measured by xanthine oxidase assay. Results Middle and lower dosage group of nickel carbonyl were observed the activity of SOD decreased in heart, spleen and kidney tissues without statistical significance, and the high dosage group of nickel carbonyl was observed obvious SOD activity suppression. The suppression was the most serious after the exposure for 3 days, and the SOD activity was recovering gradually after the exposure for 7 days with no statistical significance. For chlorine group, the decrease of SOD activity were severe than nickel carbonyl exposed groups in heart and spleen, but the high dosage group, its SOD activity was slightly lower than negative control group. Conclusions The inhalation of nickel carbonyl could cause the decrease of SOD activity in heart, spleen, kidney and led to oxidative damage.

关 键 词：羰基镍 SOD 器官 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]