五藤胶囊的薄层色谱定性鉴别及其补骨脂素HPLC定量研究  

TLC Identification and HPLC Quantitative Study of Psoralen in Wu Teng Capsules

作　　者：李万红[1] 

机构地区：[1]海安县中医院,江苏海安226600

出　　处：《西部中医药》2015年第6期25-29,共5页Western Journal of Traditional Chinese Medicine

摘　　要：目的:制定五藤胶囊的定性定量标准。方法:采用薄层色谱法,分别以化学对照品(芒丙花素、青藤碱、补骨脂素)、药材(鸡血藤、青风藤、补骨脂)为对照,鉴别样品中鸡血藤、青风藤、补骨脂等主要组成药物,并采用HPLC法测定其中补骨脂素的含量。结果:样品薄层色谱分别与化学对照品及对照药材薄层色谱在相应位置上显相同颜色的斑点。HPLC法测定样品中补骨脂素的含量,暂定胶囊内容物中补骨脂素的含量不得低于3 mg/g。结论:定性鉴别和定量分析为五藤胶囊的质量控制提供了科学依据。Objective： To establish qualitative and quantitative standards for WuTeng capsules. Methods： TLC was adopted, chemical reference substances （formononetin, diversine and psoralen） and herbs [JiXueTeng（Millettia dielsiana）, QingFengTeng （Caulis Sinomenii） and BuGuZhi （Psorslea corylifolia L.）] were taken as the references respectively, identification samples mainly contained JiXueTeng, QingFengTeng and BuGuZhi, the contents of psoralen were determined by HPLC. Results： TLC of the samples respectively showed the spots in the same color at the corresponding position compared with TLC of chemical reference substances and herbs. The content of psoralen was no less than 3mg/g in provisional capsule contents determined by HPLC method. Conclusion： Qualitative identification and quantitative analysis could offer scientific evidence for quality control of WuTeng capsules.

关 键 词：五藤胶囊 芒丙花素 青藤碱 补骨脂素 薄层色谱鉴别 HPLC法 

分 类 号：R284.1[医药卫生—中药学]