针灸预处理对摔跤运动员大负荷训练阶段IFN-γmRNA和IL-4mRNA表达的调节  

Effect of Acupuncture and Moxibustion Pretreatment on IFN-γmRNA and IL-4mRNA Expressions of Wrestlers during Heavy Load Training

作　　者：高明[1] 戴学名 吴瑛[3] 盛泽田 徐刚[1] 唐文超[1] 曹志超[5] 李稚[5] 

机构地区：[1]上海中医药大学针灸推拿学院,上海201203 [2]上海体育学院附属竞技体校,上海200438 [3]上海体育学院研究生处,上海200438 [4]安徽体育局摔柔中心,安徽合肥230001 [5]上海体育学院体育教育训练学院,上海200438

出　　处：《中华中医药学刊》2015年第7期1564-1567,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81202749);高等学校博士学科点专项科研基金博导类课题(20113156110001);上海市体育局科研攻关与科技服务课题(10JT047)

摘　　要：目的:通过观察大负荷训练期间男子摔跤运动员IFN-γmRNA和IL-4mRNA表达水平的变化,探讨针灸预处理方法防治运动性免疫失衡的可能作用机制。方法:20例摔跤运动员,随机分为对照组(10例)和实验组(10例),在8周大负荷训练期间进行实验,分别在大负荷运动训练前,大负荷运动训练后、调整训练后采取外周静脉血共5次,采用实时荧光定量PCR检测外周血γ-干扰素(IFN-γ)和白介素-4(IL-4)细胞因子mRNA的表达量。结果:摔跤运动员IFN-γmRNA表达变化,对照组W8与同组W2和W5相比明显降低(P<0.05),调整后W9明显高于W8(P<0.01);实验组W9高于同组W2和W5(P<0.05),实验组W8与同期对照组相比明显升高(P<0.05)。IL-4 mRNA表达对照组W4、W5、W8、W9均高于同组W2(P<0.05,P<0.01);实验组W5与同组W2、W4相比明显升高(P<0.01),W8、W9与同组W5相比明显恢复(P<0.05),实验组W4、W8与同期对照组相比有统计学意义(P<0.01、P<0.05)。IFN-γmRNA/IL-4mRNA比值变化,对照组W4、W5、W8均低于同组W2(P<0.05,P<0.01);实验组W5明显低于同组W2(P<0.05),W8、W9与W5相比明显恢复(P<0.05),实验组W8和W9与同期对照组相比有统计学意义(P<0.01)。结论:大负荷训练期间运动员通过针灸预处理方法调节IFN-γ和IL-4细胞因子mRNA的表达水平,进而维持运动员Th1/Th2细胞因子的动态平衡,这可能是针灸延缓或防止运动员免疫机能下降或失衡的机制之一。Objective ： To evaluate acupuncture and moxibustion pretreatment method for the changes on IFN - γmRNA and IL - 4mRNA expressions in male wrestlers after heavy load training and to explore the mechanism of the exercise - in- duced immune imbalance. Method： Twenty healthy male wrestlers were randomly divided into control group （10） and ex- perimental group （10） ,taking a heavy load training period for eight weeks. The blood sample was collected before （W2） and after （ W4, W8 ） heavy load training and after adjusting training （ W5, W9 ）. The changes of IFN - γmRNA and IL - 4mRNA expressions were evaluated by real - time fluorescent quantitative PCR. Result ： The level of IFN - γmRNA ex- pression in control group at W8 compared with that at W2 and W5 significantly decreased （ P 〈 0.05 ） and the exoerimen-tal group＇s level of IFN - γmRNA expression at W9 compared with that at W2 and W5 significantly decreased （ P 〈 0.05 ）. Experimental group compared with control group at W8 had significant difference （ P 〈 0.05 ）. The level of IL - 4 mRNA expression in control group at W4, W5, W8 and W9 compared with that at W2 was significantly higher （ P 〈 0.05, P 〈 0.01 ）. The experimental group＇s level of IL -4 mRNA expression at W5 compared with that at W2 and W4 was sig- nificantly higher （ P 〈 0.01 ）. Experimental group compared with control group at W4 and W8 had significant difference （ P 〈 0.01, P 〈 0.05 ）. IFN - γmRNA/IL - 4mRNA ratio changes in control group at W4, W5 and W8 compared with that at W2 were significantly lower （P 〈0.05 ,P 〈0.01 ） and the experimental group＇s changes at W5 compared with that at W2 was significantly different （ P 〈 0.05 ）. Experimental group compared with control group at W8 and W9 had significant difference （P 〈 0. 01, P 〈 0.05 ）. Conclusion： Wrestlers during heavy load training through the acupuncture and moxibus- tion preconditioning adjust IFN -＇y and IL -4 cytokine mRNA expression level

关 键 词：针灸预处理 大负荷训练 IFN-γmRNA IL-4MRNA 运动员 

分 类 号：R245[医药卫生—针灸推拿学]