消渴平合剂对STZ诱导的糖尿病肾病大鼠TGF-β_1/Smad7信号转导通路的影响研究  

Research of Xiaokeping Mixture in STZ-induced Diabetic Nephropathy Rats and the TGF-β_1/Smad7 Signal Transduction Pathway

作　　者：辛传伟[1] 杨秀丽[1] 田云龙[1] 李功华[1] 林蒙蒙[1] 

机构地区：[1]浙江省中医药研究院,浙江杭州310012

出　　处：《中华中医药学刊》2015年第7期1673-1675,I0010,I0011,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(LY14H280003);浙江省中西医结合学会临床药学科研基金项目(2013LYZD008)

摘　　要：目的:观察消渴平合剂对链脲佐菌素(STZ)诱导的糖尿病肾病(DN)大鼠TGF-β1/Smad7信号转导通路的影响,探讨其对DN的防治作用及机制。方法:复制DN大鼠模型,随机分为正常组10只,模型组10只、厄贝沙坦组10只和消渴平合剂组10只。12周时处死大鼠,观察各组大鼠24 h尿蛋白变化情况,检测血清尿素氮(BUN)、血肌酐(Scr)、甘油三酯(TG)、一氧化氮(NO),大鼠肾组织行HE染色,免疫组化检测肾组织TGF-β1及Smad7蛋白。结果:消渴平合剂可以改善DN大鼠的一般状况,明显降低24 h尿蛋白定量(P<0.01),减轻肾组织病理性损害。与模型组比较,消渴平合剂组BUN、Scr、TG含量显著降低(P<0.01),NO含量明显升高(P<0.01);肾组织TGF-β1蛋白表达明显降低(P<0.01),Smad7蛋白表达显著升高(P<0.01)。结论:消渴平合剂可用于治疗糖尿病肾病,其机制可能与抑制DN大鼠肾脏TGF-β1/Smad7信号转导通路的激活有关。Objective：To explore the mechanism of Xiaokeping mixture on diabetic nephropathy rats （DN rats） induced by streptozotocin （STZ）and the TGF- β1/Smad7 signal transduction pathway. Methods ：The experimental rats were ran- domly divided into four groups ： the normal control group （ n = 10 ）, the model group （ n = 10 ）, the Irbesartan group （ n = 10 ） and the Xiaokeping mixture group（ n = 10 ）. Some biochemical indicators, quantitation of urine protein in 24 hours were observed. Rats were executed on the 12th week. BUN,Scr,TG and NO in the blood serum were tested. The HE staining ways were applied to the kidney tissues. The renal proteins of TGF - β1 and Smad7 were detected. Results ： The general condition of rats could be improved by Xiaokeping mixture. The 24 hours urinary protein was signicantly reduced （ P 〈 0.01 ）. Pathological damage of kidney could be relieved. Compared with the model group, the contents of BUN, Scr, TG could be obviously reduced （P 〈 0.01 ） , and NO increased apparently （P 〈 0.01 ） ;the expressions of TGF - ~lwere signi cantly reduced （ P 〈 0.01 ）, while Smad7 could be increased （ P 〈 0.01 ）. Conclusion ： It was found that Xiaokeping mixture could be used to treat DN and the mechanism may be related to restraining the activation of TGF - β1/Smad7 signaling pathway.

关 键 词：消渴平合剂 糖尿病肾病 TGF-β1 SMAD7蛋白 

分 类 号：R285.5[医药卫生—中药学]