人参皂苷Rg1对Aβ_(1-42)诱导PC12细胞自噬性死亡的保护作用  

The Protective Effects of Ginsenoside Rg1 on Autophagy Against Aβ-induced Neurotoxicity in PC12 Cells

作　　者：陈红淑[1] 杨元宵[2] 冯耀荣[3] 许亚萍[1] 郑敏霞[1] 

机构地区：[1]浙江中医药大学附属第一医院,浙江杭州310006 [2]浙江中医药大学药学院,浙江杭州310053 [3]浙江中医药大学附属第二医院,浙江杭州310005

出　　处：《中华中医药学刊》2015年第7期1679-1681,I0012,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅项目(Y201431211);全国名老中医药专家传承工作室建设项目

摘　　要：目的:观察人参皂苷Rg1对β-淀粉样蛋白(beta-Amyliod,Aβ1-42)诱导PC12细胞自噬性死亡的作用,试探究其神经保护机制。方法:将PC12细胞分为对照组、Aβ组和Aβ+Rg1组。Aβ组细胞用Aβ1-42(10μmol/L)处理24 h;Aβ+Rg1组细胞分别用12.5、25、50μmol/L Rg1预处理24 h后,加入Aβ1-42(10μmol/L)继续培养24 h。采用MTT法测定各组细胞活力,免疫荧光染色法检测细胞自噬相关蛋白LC3-Ⅱ表达变化,Western blot法检测LC3-Ⅱ和Beclin-1蛋白表达变化。结果:对照组比较,Aβ组细胞活力显著下降(P<0.01);与模型组比较,Rg1各剂量组细胞活力显著高于Aβ组(P<0.01)。与对照组比较,Aβ组LC3-Ⅱ和Beclin-1蛋白表达显著上升(P<0.01);与模型组比较,Rg1各剂量组LC3-Ⅱ和Beclin-1蛋白表达显著低于Aβ组(P<0.01)。结论:人参皂苷Rg1可能通过抑制Aβ诱导的细胞自噬性死亡发挥神经保护作用。Objective：Ginsenoside Rgl ,one of the tfiologieally active ingredients of ginseng,may be a candidate neuro- protective drug. In the present study,the meehanism underlying the neuroproteetiun provided by ginsenosde Rgl was stud- led against aulophagy induced by Aβ1 -42 in PC12 eells. Method： PC12 cells were divided into Aβ group, Aβ ＋ Rgl group and e.ontrol group. Ceils in Aβ group were treated with Aβ1 -42 （ 10 μmol/L） for 24 h, and cells in Rgl group were pre - incubated with Rgl （ 12.5,25,50 μ mol/l,） for 24 h prior to treatment with 10 μmol/L Aβ1 -42 for 24 h, Cell viability was detected by MTT;The protein expression of LC3 - Ⅱ protein was assayed by lmmunotlnoreseence; Western blotting was used to detect the expression of LC3 - 11 and Beclin - 1. Results：The results showed that the viability of PCI2 cells in Aβ group was significantly lower than thai in control group （ P 〈 0. 01 ） , and the viability of PCI2 cells in Rgl group was significantly higher than that in Aβ group（ P 〈 0.05）. Rgl effectively inhibited the expression of LC3 - lI and Beelin - I protein induced by Aβ -42. Conclusion：These results suggest that Rgl exhibits neuroproteetive effect through inhibiting Aβ- induced autophagy in PCI2 cells.

关 键 词：人参皂苷RG1 阿尔茨海默病 Β-淀粉样蛋白 自噬 

分 类 号：R285.5[医药卫生—中药学]