慢性不可预知性温和刺激对雄性大鼠生殖系统的影响  

Effects of chronic unpredictable mild stress on reproductive system in male rats

作　　者：张莹 王尚明 张斌 王丽丽 石翠格 张树成 

机构地区：[1]国家卫生计生委科学技术研究所,北京100081

出　　处：《中国计划生育学杂志》2015年第7期440-442,共3页Chinese Journal of Family Planning

基　　金：国家重点基础研究发展计划(2010CB530403);首都医科大学神经生物学国家重点学科开放课题(CMUNEURO-201403)

摘　　要：目的:研究慢性不可预知性温和刺激对雄性大鼠生殖系统的影响。方法:将SD雄性大鼠随机分为正常对照组和模型组。模型组大鼠每天给予多种低强度刺激,连续28d,构建模型。称量大鼠体重、睾丸和附睾重量。取出附睾计数精子、观察精子活率。HE染色观察大鼠睾丸组织结构。结果:与正常对照组相比,模型大鼠体重、睾丸重量、附睾重量显著减少,精子活率显著下降,a、c级精子所占比例明显下降;组织形态学与对照组相比,模型组大鼠睾丸组织受到明显损伤。结论:慢性不可预知性温和刺激可损伤雄性大鼠的生殖功能。Objective：To explore the effects of chronic unpredictable mild stress（UCMS）on reproductive system in male rats.Methods：SPF SD rats were randomly divided into normal group and UCMS group.Rats in the UCMS group were given 28 days of chronic unpredictable mild stress,while those in the control group was normal fed.Body weight as well as the weight of testis and epididymis was measured.The number of sperm,sperm survival rate and sperm activity were evaluated.HE staining was utilized for observing the structure of rat testis.Results：Compared with the control group,the weight of testis and epididymis and the body weight of rats in UCMS group were remarkably decreased.The sperm activity decreased significantly,the rate of grade a and c sperm decreased significantly in UCMS group.The structure of testis in UCMS group was damaged compared with the control group.Conclusion：The chronic unpredictable mild stress can be harmful to the reproductive function of male rats.

关 键 词：慢性不可预知性温和刺激 睾丸 附睾 精子计数 精子活率 大鼠 

分 类 号：R698[医药卫生—泌尿科学]