培养基对小麦单倍体胚成苗率的影响  

Effect of Culture Media on Seedling Rate of Wheat Haploid

作　　者：高艳 唐建卫 殷贵鸿 韩玉林 黄峰 王丽娜 于海飞 李楠楠 吕永军 张倩 李顺成 杨光宇 李新平 

机构地区：[1]周口市农业科学院/河南省小麦种质改良工程技术研究中心,河南周口466000

出　　处：《中国农学通报》2015年第21期107-110,共4页Chinese Agricultural Science Bulletin

基　　金：河南省杰出青年基金项目"超高产;多抗;优质;高白度小麦新品种培育与开发"(144100510004);国家863计划项目"优质高产多抗小麦分子育种与品种创制"(2012AA101105);河南省现代农业产业技术体系建设专项"河南省小麦产业技术体系"(2130199-ny);转基因生物新品种培育重大专项课题"高产转基因小麦新培育"(2011ZX08002-003);国家农业科技成果转化项目"超高产稳产广适优质中筋小麦新品种国审周麦27号中试与示范"(2013GB2D000298)

摘　　要：为筛选出适宜小麦单倍体幼胚生长的培养基,提高小麦单倍体胚成苗率,将小麦×玉米远缘杂交得到的小麦单倍体幼胚接种在8种培养基上,每种培养基上接种2个材料,平均值用于数据分析。8种培养基中幼苗平均产生率分别为:46.07%、45.90%、34.11%、41.15%、37.99%、36.91%、37.70%和52.51%。结果表明1/2MS+1.0 mg/L KT+3.0 mg/L多效唑+100 mg/L肌醇+5%蔗糖+0.6%琼脂,p H 5.8培养的幼胚成苗率最高,白化苗率低,且单倍体苗的长势较好,有利于培育壮苗。以1/2MS为基本培养基,加入微量的多效唑、KT、肌醇以及较高浓度的蔗糖可以有效促进幼胚生长。In order to select the optimal culture medium for the growth of wheat haploid immature embryo andimprove the seedling rate, wheat haploid immature embryos derived from wheat × maize cross were inoculatedin eight mediums. Each medium was inoculated two materials. Mean values of the two materials were used fordata analysis. The frequency of seedling production in eight mediums were 46.07%, 45.90%, 34.11%, 41.15%,37.99%, 36.91%, 37.70% and 52.51% respectively. The results showed that the optimum culture medium wasp H5.8 medium of 1/2MS, 1.0 mg/L KT, 3.0 mg/L PP333, 100 mg/L inose, 5% sucrose and 0.6% agar. In theoptimum culture medium, the immature embryo seedling rate was the highest, albino seedling rate was low andthe haploid plantlets grew well. The basic culture medium of 1/2MS, added with trace amount of PP333, KT,inositol and high concentration sucrose, can effectively promote the embryo growth.

关 键 词：小麦 单倍体 培养基 激素 成苗率 

分 类 号：S512.1[农业科学—作物学]