小麦花粉离体萌发培养体系的筛选  

Screening of Pollen Germination in vitro Cultivation System for Wheat(Triticum aestivum L.)

作　　者：侯立江[1] 宋瑜龙[1] 牛娜[1] 马守才[1] 宋亚珍[1] 王强[1] 张改生[1] 王军卫[1] 

机构地区：[1]西北农林科技大学农学院/国家杨凌农业生物技术育种中心/国家小麦改良中心杨凌分中心/小麦育种教育部工程研究中心/陕西省作物杂种优势研究与利用重点实验室,陕西杨凌712100

出　　处：《中国农学通报》2015年第21期111-114,共4页Chinese Agricultural Science Bulletin

基　　金：西北农林科技大学基本科研业务费项目"SQ-1杀雄剂诱导小麦雄性不育过程中重要功能基因的克隆;表达和功能分析"(QN2011003);唐仲英育种基金"化杀强优势组合选育"

摘　　要：为了准确检测小麦花粉活性,以5个常规小麦品种为材料,4种花粉离体萌发培养体系为候选方法,通过比较不同体系花粉萌发率差异,从而找到最佳的小麦花粉离体萌发培养体系。结果表明:小麦成熟花粉在20%蔗糖+10%PEG4000+40 mg/L H3BO3+3×10-3mol/L Ca(NO3)2+10 mg/L VB1中28℃培养30 min,通过镜检观察,可诱导萌发频率高达90%以上。同时,经过K型小麦不育系、保持系和F1材料的鉴定,表明上述培养基适合于小麦花粉离体萌发,能够准确检测小麦花粉活性。In order to accurately detect wheat pollen activity, five conventional wheat varieties and fourcandidate liquid medium were chosen as the study materials, and the experiment was conducted to select amore excellent and suitable wheat pollen germination system in vitro culture. Results showed that thegermination rate of wheat mature pollen was induced up to more than 90% under 20% sucrose＋20% PEG4000＋40 mg/L H3BO3＋3×10-3mol/L Ca（NO3）2＋10 mg/L VB1 at 28℃ for 30 min. Meanwhile, the identification resultsof the three series of wheat, including K-type wheat sterile line, maintainer line and F1 materials, showed thatthe medium was more suitable for in vitro pollen germination of wheat, and was able to accurately detect wheatpollen activity. At the same time, the study could provide certain theoretical basis for the preservation of wheatpollen and wheat breeding.

关 键 词：小麦 花粉粒 离体萌发 花粉管 

分 类 号：S-3[农业科学]