甘蔗物理诱变M2代株系的ISSR分析  

ISSR Analysis of Physically-mutated Sugarcane Strains in M2 Generation

作　　者：廖芩[1] 黄河星 黄兴[1] 杨善[1] 莫俊杰[1] 周鸿凯[1] 

机构地区：[1]广东海洋大学农学院,广东湛江524088

出　　处：《江西农业学报》2015年第7期28-30,36,共4页Acta Agriculturae Jiangxi

基　　金：国家自然科学基金项目(41073059);广东省科技计划项目(2013B020301005;2013B020301008)

摘　　要：应用ISSR标记技术研究了海大32及其物理诱变M2代33个株系的遗传多样性。结果表明：海大32及其M2代株系的相似水平在0.86~1.00之间;在0.94左右的水平上,可以将它们分为4个大类,其中海大32及海大10-1等25个株系为第一类,海大10-3、海大10-27等6个株系聚为第二类,海大10-33单独为第三类,海大10-20和海大10-21聚为第四类;海大10-20、海大10-21发生变异的程度最大。The author applied ISSR technique to study the genetic diversity of sugarcane variety Haida 32 and its physically- mutated 33 strains in M2 generation. The results showed that the similarity between Haida 32 and its M2-generation strains varied from 0.86 to 1.00. At the level of 0.94 or so, they could be divided into 4 types, the first type included 25 strains （ Haida 32, Haida 10-1, and so on）, the second type included 6 strains （Haida 10-3, Haida 10-27, etc.）, the third type only included Haida 10- 33, and the fourth type included Haida 10-20 and Haida 10-21. After physical mutation, the greatest degree of variations occurred in Haida 10-20 and Haida 10-21.

关 键 词：甘蔗 ISSR 物理诱变 株系 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]