基因型和预处理对非洲菊胚珠离体培养的影响  

Effects of Genotype and Pretreatment on Isolated Culture of Ovule in Gerbera jamesonii

作　　者：单芹丽[1] 李绅崇[1] 王继华[1] 吴丽芳 汪国鲜 王丽花[1] 吴旻[1] 杨春梅 

机构地区：[1]云南省农业科学院花卉研究所云南省花卉育种重点实验室国家观赏园艺工程技术研究中心,云南昆明650205 [2]玉溪云星生物科技有限公司,云南玉溪652604

出　　处：《江西农业学报》2015年第7期44-47,共4页Acta Agriculturae Jiangxi

基　　金：云南省战略性新兴产业发展专项资金项目(昆财企一[2013]118号);云南省农业科学院院创新团队专项(YAAS2014TD002);云南省科技厅科技创新强省计划(2014AB014)

摘　　要：以非洲菊胚珠为试材,研究了基因型、低温和培养前期黑暗处理(暗培养)对非洲菊胚珠培养的影响。结果表明:对愈伤组织诱导率和不定芽诱导时间影响的主次关系均为基因型>暗培养>低温,而对不定芽诱导率影响的主次关系是基因型>低温处理>暗培养。基因型是决定诱导效果的关键因素;低温处理7 d,愈伤组织和不定芽的诱导率最高,但低温处理对不定芽的诱导时间没有显著影响;暗培养21 d,愈伤组织的诱导效果最好;暗培养14 d,不定芽的诱导效果最好。The effects of genotype, low temperature and dark culture on the isolated culture of ovule in Gerbera jamesonii were studied. The results indicated that the effect of 3 factors on both callus induction rate and adventitious bud induction time had the fol- lowing order： genotype〉 dark culture〉 low temperature, while that on induction rate of adventitious buds showed the following se- quence ： genotype〉 low temperature 〉 dark culture. Gerbera genotype was a key factor determining the induction effect of ovule cul- ture. Low temperature treatment for 7 d achieved the highest induction rate of both callus and adventitious buds, but low temperature treatment had no significant effect on adventitious bud induction time. Dark culture for 21 d obtained the best induction effect of callus, and dark culture for 14 d achieved the best induction effect of adventitious buds.

关 键 词：非洲菊 胚珠 基因型 低温 暗培养 

分 类 号：S682.11[农业科学—观赏园艺]