苯并[a]芘暴露对绦柳生长发育的影响  

Effects of Benzo[a]pyrene on the growth and development of Salix matsudana f.pendulaSchneid.

作　　者：孙然[1,2] 池翠兰 李燕玲[1] 杜克久[1,2] 

机构地区：[1]河北农业大学,河北保定071001 [2]河北省林木种质资源与森林保护重点实验室,河北保定071001 [3]河北省大海陀自然保护区,河北赤城075500

出　　处：《河北林果研究》2015年第2期126-128,共3页Hebei Journal of Forestry and Orchard Research

基　　金：国家高技术研究发展计划课题"863计划""农田有机复合污染的控制与修复技术"(2012AA101403);中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室开放基金课题"芦苇降解环境POPs功能基因研究"(KF2009-03)

摘　　要：为了了解PAHs植物修复机制,利用绦柳组织培养体系,研究了在离体条件下MS培养基添加不同浓度BaP的单独暴露,以及MS+0.1mg/L 6-BA+0.2mg/L NAA不定根分化培养基添加不同浓度BaP的协同激素暴露对绦柳生长发育的影响。结果发现,在设计的暴露剂量范围内,MS添加不同浓度BaP的单独处理可显著促进绦柳不定根的分化,4.0mg/L BaP单独暴露条件下绦柳的初根时间比阴性对照CK-提前了2d,不定根分化率达100%的时间比阴性对照提前了2d,不定根的生根数量是阴性对照的1.8倍;和阴性对照CK-相比,各暴露剂量条件下绦柳根茎叶的生物量变化不明显;MS+0.1mg/L 6-BA+0.2mg/L NAA并添加不同浓度BaP的协同激素处理,在设计的暴露剂量范围内各处理绦柳均没有不定根的分化,但切口部位有愈伤组织的形成;和阳性对照CK+相比,各暴露剂量条件下绦柳茎叶的生物量变化不明显。Effects of Benzo[a]pyrene on the growth and development of Salix matsudana were studied through adding the different doses of BaP in MS media or in media of MS＋0.1mg/L 6-BA＋0.2 mg/L NAA.The results showed that BaP affected the differentiation of adventitious roots in MS media.4.0mg/L BaP caused the maximum effect on the formation time of an initial root which was 2dearlier than the negative control（CK-）,and its period to reach the 100%of adventitious root differentiation rate was also 2dearlier than CK- with the adventitious root amount being 1.8times as high as CK-.However,BaP with various doses added in MS media did not play any effect on the stem and leaf biomass compared to the CK-.BaP in media of MS＋0.1mg/L 6-BA＋0.2mg/L NAA resulted in the calli formation instead of adventitious roots differentiation and insignificant effects on the stem and leaf biomass compared to the CK＋.

关 键 词：苯并[A]芘 绦柳 不定根分化 生长发育 生物量 

分 类 号：S792.12[农业科学—林木遗传育种]