苦参、茜草对溃疡性结肠炎大鼠黏膜损伤修复作用的影响  

Effects of Sophora Flavescens and Rubia Cordifolia on Mucosal Repair of Ulcerative Colitis Rats

作　　者：王玖[1] 沈洪[2] 朱磊[2] 张露[2] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]江苏省中医院,江苏南京210029

出　　处：《湖北中医药大学学报》2015年第3期10-13,共4页Journal of Hubei University of Chinese Medicine

基　　金：国家中医药行业科研专项资助项目(201407001);国家自然科学基金资助项目(81403344;81373606);江苏省自然科学基金资助项目(BK2011078);国家中医药管理局项目(JDZX2012079);江苏省中医院院级课题项目(Y13051);江苏高校优势学科建设工程资助项目(PAPD);国家中医临床研究基地(脾胃)资助项目;江苏省中医消化病临床医学研究中心建设项目(BL2014100)

摘　　要：目的观察中药苦参、茜草对2,4,6-三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎(UC)大鼠模型结肠黏膜形态的影响,对其黏膜损伤修复机制进行探讨。方法采用苦参、茜草及西药柳氮磺胺吡啶(SASP)对UC大鼠模型进行治疗,治疗结束后采集结肠黏膜标本,首先肉眼观察结肠黏膜组织,按组织损伤程度进行大体形态评分,组间比较分析。然后将结肠组织石蜡包埋,HE染色,光镜下进行观察其黏膜病理学变化。结果模型组大鼠结肠黏膜损伤与正常组间差异极显著(P<0.01)。与模型组相比,苦参组、茜草组、SASP组大鼠体质量显著增加,但3组间无明显统计学差异。苦参组、茜草组、SASP组大鼠结肠大体形态损伤水平及病理学评分均明显低于模型组,但3组间无明显统计学差异。结论苦参、茜草、柳氮磺胺吡啶均能有效增加溃疡性结肠炎大鼠体质量,使UC大鼠结肠溃疡个数及面积明显减少,抑制炎性细胞浸润,使水肿、糜烂等病理改变得到明显改善,表明苦参、茜草对TNBS诱导的UC大鼠有较好的治疗作用。Objective To investigate the effects of Sophora flavescens and Rubia cordifolia in the colonic mucosa repairing of 2,4,6 -trinitrobenzenesulfonic acid（TNBS） -induced ulcerative colitis （UC） in rats.Methods We used Sophora flavescen, Rubia cordifolia and SASP to treat rat UC model.After the treatment,colonic mucosa specimens were collected to describe the damagement ,and compare in groups.Then the colon mucosa which was paraffin -embed, HE -stained was observed under light microscope .Results Colonic mucosa in-jury in rats model group was significant different between the normal group （P〈0.01）.Compared with model group, the body mass of the treatment group rats increased significantly , but there was no statistical significant difference among three groups .The colon damage level and pathology score of treatment group rats were significantly lower than the model group , no statistical significance between the three groups . Conclusion Sophora flavescen, Rubia cordifolia and SASP can effectively increase the CU rats ’ body mass, which also make the colon ul-cer number and area reduced significantly .The pathological changes such as edema , erosion dramatically improve.Sophora flavescen and Rubia cordifolia have better treatment in TNBS induced UC rats .

关 键 词：溃疡性结肠炎 苦参 茜草 黏膜修复 

分 类 号：R285.5[医药卫生—中药学]