广西地方禽类品种ALV和REV感染情况的调查及ALV分离株gp85基因序列分析  

Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates

作　　者：钟兴福[1,2] 王培坤[1] 杨永立[1] 秦丽莉[1] 毕玉彧[1] 邹广珍[1] 彭昊[1] 韦平[1] 

机构地区：[1]广西大学养禽与禽病学研究所,南宁530004 [2]广西钦州市钦南区沙埠镇水产畜牧兽医站,钦州535000

出　　处：《中国畜牧兽医》2015年第7期1647-1653,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家公益性(农业)行业科研专项(201203055);国家现代农业产业技术体系广西肉鸡产业创新团队建设项目(nycytxgxcxtd-04-20-2)

摘　　要：为了解广西钦州地区主要地方品种商品禽类中禽白血病病毒（ALV）、禽网状内皮组织增殖症病毒（REV）的感染情况，本试验随机采集了广西钦州地区代表饲养场的麻鸭、狮头鹅、铁脚麻鸡、火鸡、鸽子共5个品种的蛋清、肛拭子及血清样品共953份，使用ELISA商品试剂盒进行检测；然后对部分ALVp27阳性的个体采集其血清样品接种DF-1细胞进行病毒分离并测定其gp85基因的序列。结果发现，除铁脚麻鸡外其他4个非鸡禽类品种的ALV检测均为阴性；除鸽子和狮头鹅外，麻鸭、铁脚麻鸡、火鸡均检测到REV抗体阳性；获得的两株铁脚麻鸡ALV分离株gp85基因之间核苷酸同源性为94．5％，与参考株之间核苷酸同源性为86．9％～94．9％；两株分离株gp85基因之间氨基酸同源性为91．5％，与参考株之间氨基酸同源性为84．0％～91。6％。高变区hrl和hr2区存在较多可变位点，可变区vr2和vr3相对较保守。系统进化树分析结果表明这两个毒株与参考株SCAUll-XG的亲缘关系最近。In order to investigate the infection status of avian leukosis virus （ALV） and avian reticuloendotheliosis virus （REV） in the major local breeds of Qinzhou,Guangxi,totally 953 sam- ples of egg white, cloaca swab and serum of Ma duck, Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits. ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced. The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken, while REV antibody was found positive in Ma duck, Tiejiao- Ma chicken and turkey. The nucleotide sequences of gp85 gene of two isolates shared 94. 5% identity with each other, and shared 86. 9% to 94. 9% with reference strains. The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6 % with reference strains. There were many variable sites in the hyper variable region hrl and hr2, and the vr2 and vr3 variable regions were relatively conservative. Phylogenetic tree analy- sis showed that the two isolates shared the highest homology with SCAUll-XG strain.

关 键 词：禽类 禽白血病 禽网状内皮组织增殖症 流行病学调查 GP85基因 系统进化树分析 

分 类 号：S852.65[农业科学—基础兽医学]