碱性成纤维细胞生长因子对牛骨骼肌卫星细胞增殖的影响  

Effect of Basic Fibroblast Growth Factor on the Proliferation of Bovine Skeletal Muscle Satellite Cells

作　　者：张蔚然[1] 代阳[1] 王轶敏[1] 刘新峰[1] 王婷[1] 郭宏[1] 丁向彬[1] 

机构地区：[1]天津农学院动物科学与动物医学学院,天津300384

出　　处：《中国畜牧兽医》2015年第7期1763-1769,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31201021);天津市自然科学基金(13JCQNJC14600)

摘　　要：为研究碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对牛骨骼肌卫星细胞增殖的影响,试验对牛骨骼肌卫星细胞进行体外分离培养,在生长培养基和分化培养基中分别添加bFGF,观察细胞生长及分化情况,绘制细胞生长曲线,并进行EDU细胞增殖检测试验。结果显示,添加了bFGF培养基的细胞生长状态较对照组良好,细胞增殖速度快,细胞增殖率显著高于对照组(P<0.05),说明bFGF对牛骨骼肌卫星细胞在生长培养基和分化培养基中增殖都具有良好的促进作用。本研究建立了一种高效的牛骨骼肌卫星细胞培养方案,为骨骼肌卫星细胞的研究利用提供参考。To investigate the effect of basic fibroblast growth factor （bFGF） on the proliferation of bovine skeletal muscle satellite cells, bovine skeletal muscle satellite cells were isolated and cul- tured in the medium with bFGF, the growth and differentiation state of muscle satellite cells were observed,and the growth curve and EDU cell proliferation assay were conducted. The results showed that cell morphology of bovine skeletal muscle satellite cells cultured in medium contai- ning bFGF was better and proliferation rate was significantly higher than that in control group （P〈0.05）. The results indicated that bFGF could promote proliferation of bovine skeletal muscle satellite cells efficiently in growth medium and differential medium. Our study established an effi- cient method to culture bovine skeletal muscle satellite cells, which could provide reference for studying and using of skeletal muscle satellite cells.

关 键 词：牛 骨骼肌卫星细胞 碱性成纤维细胞生长因子 细胞增殖 

分 类 号：S823[农业科学—畜牧学]