GPR54基因在绵羊不同组织中的表达及其在睾丸中的定位研究  

Expression of GPR54 Gene in Different Tissues and Cellular Localization in Testis of Sheep

作　　者：路佩瑶[1] 王福传[1] 宋献艺[1] 张凯[1] 张宁[1] 岳文斌[2] 

机构地区：[1]山西省农业科学院饲料兽药研究所,太原030031 [2]山西农业大学动物科技学院,太谷030801

出　　处：《中国畜牧兽医》2015年第7期1816-1822,共7页China Animal Husbandry & Veterinary Medicine

基　　金：山西省农业科学院博士基金项目(YBSJJ1403);现代农业产业技术体系建设专项资金(NYCYTX-39)

摘　　要：试验旨在研究GPR54（G-protein coupled receptor54）基因在绵羊不同组织中的表达及其在4～6月龄公羔睾丸组织中的表达特性及定位。本研究采用实时荧光定量PCR法检测各组织GPR54mRNA表达规律，以及其在不同月龄公羔睾丸中的表达量，运用免疫组化技术对睾丸中GPR54基因进行定位分析。结果显示，GPR54在不同组织中均有不同程度的表达，其中在下丘脑、垂体和睾丸中大量表达；GPR54在4～6月龄绵羊公羔睾丸组织中呈阶段性表达，6月龄表达量显著高于4和5月龄（P〈0．05）；4月龄时，在精原细胞和极少数的初级精母细胞中检测到较弱的阳性信号；6月龄性成熟时，在睾丸中分裂早期的精子细胞检测到强阳性信号。综上表明，GPR54基因在绵羊不同组织中广泛表达，在下丘脑一垂体一睾丸生殖轴中发挥重要的作用，并与动物的性成熟及雄性动物精子发生过程有密切的关系。The objectives of this study were to evaluate the expression of G-protein coupled recep- tor 54 （GPR54） in various tissues and the cellular localization of testis of 4 to 6-month-old sheep. In this experiment,mRNA expression was detected by Real-time fluorescence quantitative PCR. Cellular localization of GPR54 in testis was examined by immunohistochemistry. The results showed that GPR54 mRNA was expressed in all the tissues,and abundantly expressed in hypo- thalamus, pituitary gland and testis. The expression level gradually increased with the individual growth and development, the mRNA and protein expression of GPR54 in testis of 6-month-old was significantly higher than 4-month-old and 5-month-old （P （0.05）;It was detected that GPR54 expression only in spermatogonia and a very number of primary spermatocytes in the tes- tis by immunohistochemical staining, and it could detect significantly that the GPR54 expression in many split early sperm cells. The results demonstrated that it had close relationship between GPR54 and sexually mature of animals and the process of spermatogenesis in male animals.

关 键 词：GPR54基因 绵羊 实时荧光定量PCR 免疫组化 睾丸 

分 类 号：S814.8[农业科学—畜牧学]