一株山羊魏氏梭菌的分离鉴定及其致病性研究  

Isolation,Identification and Pathogenicity Research of a Strain of Clostridium perfringens from Goat

作　　者：孟霞 孙翠平 唐娜[1,2] 苗立中[1,2] 沈志强[1,2] 

机构地区：[1]山东绿都生物科技有限公司,滨州256600 [2]山东省滨州畜牧兽医研究院,滨州256600

出　　处：《中国畜牧兽医》2015年第7期1905-1909,共5页China Animal Husbandry & Veterinary Medicine

基　　金：山东省现代农业产业技术体系羊产业创新团队项目(SATS-201226-3)

摘　　要：本试验从一例病死山羊组织内分离到一株致病性细菌,通过鉴别培养、生化试验、分子生物学试验和动物致病性试验,证明该病原菌为羊致病性A型魏氏梭菌;毒素基因分析结果显示,该菌同时含有α和β2两种毒素基因;动物致病性试验结果表明,该菌对昆明小鼠具有较强的致病性,并从试验致死的昆明小鼠病料中分离到了与病死山羊病原相一致的细菌,从而确定A型魏氏梭菌为引起该山羊死亡的主要病原菌。本试验结果为该羊场魏氏梭菌病的治疗和预防提供了科学依据。A pathogenic bacteria was isolated from a dead goat,and it was identified as goat-patho- genic type A Clostridium perfringens by differential culture, biochemical test, molecular biologi- cal technology and animal pathogenicity test. Analysis results of toxin genes showed that this bac- teria contained both α and β2 toxin genes. Animal pathogenicity test result showed that this bacte- ria was highly pathogenic to mice, and the same bacteria was isolated from dead mice as which was isolated from the dead goat,so it was certain that type A Clostridium perfringens was the main pathogenic bacteria which caused the goat dead. The results would provide scientific basis for the treatment and prevention of the disease caused by Clostridium perfringens in this goat farm.

关 键 词：山羊 魏氏梭菌 分离 厌氧培养 细菌学鉴定 

分 类 号：S852.616.3[农业科学—基础兽医学]