乙型肝炎患者HBV-DNA载量与TNF-α、NO及Hcy的相关性分析  

Correlative Analysis On Serum HBV-DNA Capacity And TNF-α,NO and Hcy Levels In Chronic Hepatitis B Patients

作　　者：罗建业[1] 郑定容[2] 

机构地区：[1]深圳市坪山新区人民医院中集社区健康服务中心,广东深圳518118 [2]深圳市宝安区西乡人民医院社区健康服务管理中心,广东深圳518102

出　　处：《现代诊断与治疗》2015年第6期1207-1210,共4页Modern Diagnosis and Treatment

摘　　要：目的研究乙型病毒性肝炎(HBV)患者外周血中HBV-DNA载量与外周血中、肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)及高半胱氨酸(Hcy)的相关性分析。方法随机选取360例HBV患者组治疗前后各一份血清,采用实时荧光定量PCR法检测外周血中HBV-DNA,用化学发光免疫分析法(CLIA)检测外周血中TNF-α、NO及HCY的浓度,并与正常对照组120例作比较,利用SPSS 17 for Windows软件进行相关性统计分析。结果 HBV患者组治疗前血清外周血中TNF-α、NO及HCY的浓度均明显高于正常对照组(P<0.01);HBV患者组的HBV-DNA载量与TNF-α含量相关性为:r=0.831,P<0.01;与HCY含量相关性为:r=0.856,P<0.01;与NO含量相关性为:r=0.745,P<0.01。在治疗后,HBV患者组外周血中HCY、NO及TNF-α的浓度水平都与正常对照组比较低,无显著性差异(P>0.05);HBV患者组的HBV-DNA载量与TNF-α含量相关性为:r=0.312,P>0.05;与HCY含量相关性为:r=0.235,P>0.05;与NO含量相关性为:r=0.334,P>0.05。结论 HBV-DNA阳性HBV患者血清中HBV-DNA载量与外周血TNF-α、NO及HCY含量均有良好的相关性。Objective To study the relationship among Hepatitis B virus DNA （HBV-DNA）, TNF-α, NO and Hcy levels in chronic hepatitis B patients.Methods Randomly selected 360 cases of HBV-DNA were positive in a group of patients with HBV before and after treatment the a serum, HBV-DNA was detected by Realtime-PCR,TNF-α,NO and Hcy were detected by Chemilumines-cence immunoassay （CLIA） and auto biochemistry analyzer respectively.And 120 cases of normal control group for comparison,The data was analyzed by SPSS 17 for Windows software.Results The TNF-α,NO and Hcy levels of group of patients with HBV were higher than that of normal control group （P〈0.01）,The group of patients with HBV of HBV-DNA copys TNF-αcontent relevance：r=0.831,P〈0.01;Comparative Hcy content relevance：r=0.856,P〈0.01;Comparative NO content relevance：r=0.745,P〈0.01;After treatment in patients with HBV in peripheral blood serum concentrations of TNF-α,NO and Hcy with the normal control group, no significant difference （P〉0.05）.The connec-tion between in patients with HBV group of HBV-DNA load and TNF-α：r=0.312,P〉0.05; and Hcy：r=0.235,P〉0.05;and NO：r=0.334,P〉0.05.Conclusions Serum HBV-DNA capacity related periph-eral blood TNF-α,NO and Hcy with HBV-DNA positive that was a good correlation.

关 键 词：乙型病毒性肝炎 乙肝病毒脱氧核糖核酸 肿瘤坏死因子-α 一氧化氮 N-乙酰半胱氨酸 相关性 

分 类 号：R512.62[医药卫生—内科学]