牛樟芝水溶性成分的HPCE-DAD指纹图谱研究  

Study on HPCE-DAD fingerprint of water-soluble components of Antrodia camphorata

作　　者：张奉苏[1] 陈菲[1] 傅兴圣[1] 刘训红[1] 杨念云[1] 蔡宝昌[1] 夏敏媛[1] 

机构地区：[1]南京中医药大学,南京210023

出　　处：《中华中医药杂志》2015年第7期2349-2352,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省中药炮制重点实验室开放式课题(No.ZYPZ007);江苏省高校优秀科技创新团队"中药资源化学研究"(No.2011);江苏高校优势学科建设工程资助项目(No.ysxk-2010)~~

摘　　要：目的:建立牛樟芝水溶性成分的HPCE-DAD指纹图谱分析方法。方法:采用高效毛细管电泳法进行色谱分离,以60mmol/L硼砂(p H值9.3)为运行缓冲液,分离电压为22k V,检测波长为260nm,压力进样为50mbar×6s,毛细管温度为28℃。以腺苷为参照物,测定其指纹图谱,并作模糊聚类分析和相似度评价。结果:初步建立了以10个共有峰为特征指纹信息的牛樟芝水溶性成分的HPCE-DAD指纹图谱,相似度较高,色谱分离度较好,达到了中药指纹图谱的技术要求。结论:方法准确可靠,重现性好,可用于牛樟芝内在质量的评价和控制。Objective： To establish the analytical method for the fingerprint of water-soluble components of Antrodia camphorata by HPCE-DAD. Methods： Based on the mode of high performance capillary electrophoresis, 60mmol/L sodium borate was used as buffer solution（p H 9.3）. The separation voltage was 22 k V and the detection wavelength was set at 260 nm. The sample was injected at 50mbar×6s and column temperature was maintained at 28℃. Adenosine was used as reference standard, the chromatographic fingerprints were determined. The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples. Results： HPCE-DAD fingerprints with 10 common peaks of water-soluble components of Antrodia camphorata were established preliminarily. There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint. Conclusion： The method was reliable, accurate and could be used for quality control of Antrodia camphorata.

关 键 词：牛樟芝 高效毛细管电泳二极管阵列检测法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学] O657.7[医药卫生—中医学]