尿蛋白诱导肾小管上皮细胞损伤及人参皂苷Rg1的再生作用  

Regenerative action of ginsenoside Rg1 on damaged renal tubular epithelial cells induced by urine protein

作　　者：杨汝春[1] 俞东容[1] 张娟[2] 朱晓玲[1] 林宜[1] 张迎华[1] 

机构地区：[1]杭州市中医院,杭州310007 [2]浙江中医药大学,杭州310053

出　　处：《中华中医药杂志》2015年第7期2573-2576,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然科学基金资助项目(No.Y2081014)~~

摘　　要：目的:探讨人参皂苷Rg1对尿蛋白诱导肾小管上皮细胞损伤后肾小管上皮细胞的再生作用及机制。方法:体外培养的HK2细胞,予不同浓度尿蛋白刺激,MTT法、LDH比色法观察细胞的成活及细胞膜稳定性;以人参皂苷Rg1进行干预,肝细胞生长因子(HGF)为阳性对照,用MTT法检测细胞存活率,western blot和实时定量RT-PCR检测增殖细胞核抗原(PCNA)基因及蛋白水平。结果:一定浓度的尿蛋白能够显著减少HK2细胞存活率、细胞膜稳定性(P<0.05),且存在浓度效应;浓度为3mg/m L的尿蛋白刺激下的HK2细胞PCNA蛋白和PCNA m RNA表达显著降低(P<0.05);而人参皂苷Rg1能提高尿蛋白损伤的HK2细胞存活率,且上调了PCNA蛋白的表达(P<0.05),与HGF比较无差异。结论:超载尿蛋白致体外培养的肾小管上皮细胞存活率降低,人参皂苷Rg1可提高尿蛋白损伤的肾小管上皮细胞存活率,促进细胞增殖。Objective： To investigate the regeneration mechanism of ginsenoside Rg1 on damaged renal tubular epithelial cells induced by urine protein. Methods： The HK2 cells were cultured in vitro. The cells were cultured in different concentrations of urinary protein（Upro）, and the cellular survival rate and stability of cell membrane were detected by MTT assay and lactate dehydrogenase（LDH） release method. The cells were intervened by ginsenoside Rg1, and hepatocyte growth factor（HGF） as the positive control group. Cell survival rate was tested by MTT method, and the protein and gene expression of proliferatingcellnuclearantigen（PCNA） were detected by western blot and RT-PCR. Results： Certain concentration of urinary protein could decrease HK2 cell survival rate and the stability of cell membrane（P〈0.05）, and the effect of urinary protein showed a concentration dependence. PCNA protein and m RNA expression decreased in HK2 cells obviously stimulate by urinary protein at 3mg/m L（P〈0.05）. Rg1 could increase the survival rate of HK2 cells injured by urinary protein and up-regulate the expression of PCNA protein（P〈0.05）, which had no difference when compared with HGF. Conclusion： Overload urinary protein could decrease the survival rate of renal tubular epithelial cells cultured in vitro. Ginsenoside Rg1 could increase the survival rate of injured renal tubular epithelial cells induced by urinary protein, and promote cell proliferation.

关 键 词：人参皂苷RG1 尿蛋白 肾小管上皮细胞 增殖 

分 类 号：R285[医药卫生—中药学]