HPCE测定龙柴方及组方药甘草中5种指标成分的含量  

Content determination of 5 compounds in Longchai Formula and Glycyrrhizae radix et rhizoma root by HPCE

作　　者：周逸芝[1] 刘训红[1] 陈菲[1] 张奉苏[1] 

机构地区：[1]南京中医药大学,南京210046

出　　处：《中华中医药杂志》2015年第7期2586-2589,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省科技厅"科技基础实施建设计划"专项(No.BM2009903);江苏高校优势学科建设工程(No.ysxk-2010);江苏省中药炮制重点实验室开放式课题(No.ZYPZ007)~~

摘　　要：目的:建立高效毛细管电泳(HPCE)法,同时测定龙柴方及其组方药材甘草中5种指标成分含量,控制该方药的质量。方法:40mmol/L硼砂-10mmol/L磷酸二氢钠-10%甲醇(p H=8.2)为电泳介质,未涂渍标准熔融石英毛细管(64.5cm×75μm,有效长度56cm)为分离通道,分离电压为20k V,检测波长为254nm,毛细管温度为20℃,压力进样为5k Pa×6s。结果:此方法可以使样品达到基线分离,阴性样品无干扰,5种指标成分的浓度与峰面积的线性关系良好(r>0.9950);加样回收率为95.00%-100.90%。结论:该方法分离良好、精密准确,可用于龙柴方及其组方药材甘草的质量控制。Objective： To establish a high-performance capillary electrophoresis（HPCE） method for determination of 5 compounds in Longchai Formula and Glycyrrhizae radix et rhizoma simultaneously and control the its quality. Methods： 40 mmol/L sodium borate-10mmol/L sodium dihydrogen phosphate-10% methanol was used as the electrophoretic medium（p H=8.2）. Uncoated fused silica capillary（64.5cm×75μm, effective length of 56cm） was used as separate channel with separation voltage of 20 k V, detection wavelength of 254 nm, column temperature of 20℃, and the inject pressure of 5k Pa×6s. Results： This method could make sample separate at the baseline, and it was not affected by negative samples. There was a good linear relation between concentration and peak area of 5 compounds（r〉0.9950）, and the recovery rate of sample was between 95.00% and 100.90%. Conclusion： This method is good and accurate, and could be suitable for quality control of Longchai Formula and Glycyrrhizae radix et rhizoma.

关 键 词：高效毛细管电泳法 龙柴方 甘草 指标成分 含量测定 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]