防感煎剂对甲型H1N1流感病毒感染小鼠NO、iNOS以及LYZ的作用研究  

Influence of Fanggan Decoction on NO, iNOS and Iysozyme in Mice Infected with Influenza A Virus Subtype H1N1

作　　者：曹羽[1] 杨珺超[1] 汪玉冠[1] 夏永良[1] 王媛[1] 

机构地区：[1]浙江中医药大学附属第一医院,杭州310006

出　　处：《浙江中医药大学学报》2015年第5期366-371,共6页Journal of Zhejiang Chinese Medical University

基　　金：浙江省医药卫生科技计划项目(2010KYB078)~~

摘　　要：[目的]探讨防感煎剂对甲型H1N1流感病毒感染小鼠一氧化氮(nitric oxide,NO)、诱导型一氧化氮合酶(inducible nitric oxide synthase,i NOS)以及溶菌酶(lysozyme,LYZ)的作用,进一步了解防感煎剂的疗效机理。[方法]选用清洁级雄性ICR小鼠96只,随机分为4组:正常对照组、模型对照组、防感煎剂组、达菲对照组。正常对照组和模型对照组用生理盐水灌胃,防感煎剂组按29.32g·kg-1剂量给予防感煎剂灌胃,达菲对照组按0.02g·kg-1剂量给予达菲胶囊溶液灌胃,各组给药1次/d。各组正常给药2d,第3d开始除正常对照组外均用甲型H1N1流感病毒液滴鼻造模,正常对照组用等量生理盐水滴鼻。给药持续到第8d,即造模后第5d,各组停止给药。造模后的第1、5、9、13d观察记录小鼠体重及一般情况,以上时间点每组随机处死6只小鼠,收集肺组织标本,RT-PCR检测感染小鼠流感病毒含量。取血分离血清,ELISA法检测小鼠血清NO、i NOS、LYZ含量。[结果]一般情况:模型对照组小鼠形体消瘦,毛色枯槁,精神萎靡、反应迟钝,饮食减少。防感煎剂组及达菲对照组小鼠症状明显较模型对照组减轻。体重:在第5、9、13d,防感煎剂组和达菲对照组小鼠平均体重均低于正常对照组(P<0.05),并且均高于模型对照组(P<0.01)。甲型H1N1流感病毒含量:第1、5、9、13d,防感煎剂组及达菲对照组小鼠肺组织病毒含量均低于模型对照组(P<0.05)。血清NO、i NOS、LYZ的含量:在第5d,防感煎剂组和达菲对照组小鼠血清NO、i NOS、LYZ含量明显高于模型对照组(P<0.05)及正常对照组(P<0.01),其余时间点差异无统计学意义(P>0.05)。[结论]防感煎剂可抑制甲型H1N1流感病毒复制,增强NO、i NOS以及LYZ的表达,可能通过巨噬细胞环节,达到抵御或清除病毒的目的。Objective] To explore the influence of Fanggan Decoction on nitric oxide（NO） ,inducible nitric oxide synthase（iNOS） and lysozyme（LYZ） in mice infected with Influenza virus type 1. [Methods] 96 ICR male mice were randomly divided into four groups： normal control group ,model control group, Fanggan decoction group and Tamiflu control group. Normal control group and model control group were given saline with intragastric administration , Fanggan decoction group were treated with Fanggan decoction at dose of 29.32 g·kg-1,Tamiflu control group were treated with Tamiflu capsule solution at dose of 0.02 g·kg-1, each group administered one time per day. Each group administered for two days, on the 3rd day ,the H1N1 flu virus was made into droplets to nose to establish model expecting the normal control group with the same amount of saline. We stopped administering on the 8th day. The weight of mice and general condition were observed and recorded on the 1st, 5th, 9th, 13rd day after modelling. Six mice in each group was killed randomly on the same time point. Lung tissue samples were collected for the determination of H1N1 virus content by RT-PCR, while serum was collected for testing contents of NO,iNOS,LYZ by ELISA. [Results] General： mice in model control group were peaked, color haggard, apathetic, unresponsive, eating less, while mice in Fanggan decoction group and Tamiflu control group had less symptoms than those in the model control group. Weight： On the 5th,9th, 13rd day, the average weight of mice in Fanggan decoction group and Tamiflu control group was lower than those in the normal control group（ P〈0.05）, and was higher than those in the model control group（P〈0.01）. H1N1 virus content： On the 1st,5th,9th,13rd day, mice lung tissue virus levels in Fanggan decoction group and Tamiflu control group were lower than those in the model control group （ P〈0.05）. Contents of NO, iNOS, LYZ in mice serum： On the 5th day, the contents of NO, iNOS, LYZ in mice serum in Fanggan dec

关 键 词：防感煎剂 甲型H1N1流感病毒 NO INOS LYZ 

分 类 号：R331[医药卫生—人体生理学]