The Role of NF mRNA and Calpain in NF Reduction of Acrylamide Neuropathy  

作　　者：YU Su Fang WEI Xiao Min YAN Feng Feng WANG Shu E ZHANG Cui Li YANG Xi Wei 

机构地区：[1]Department of Occupational Health and Occupational Medicine,School of Public Health,Shandong University

出　　处：《Biomedical and Environmental Sciences》2015年第6期445-448,共4页生物医学与环境科学（英文版）

基　　金：the National Natural Science Funding of China(No.30872088,81372969)

摘　　要：The purpose of this study was to study the role of neurofilament （NF） mRNA and calpain in NF reduction of acrylamide （ACR） neuropathy. Male Wistar adult rats were injected i.p. every other day with AER （20 mg/kg.bW or 40 mg/kg.bW） for 8 weeks. NF mRNA expression was detected using RT-PCR and the calpain concentration was determined using western blot analysis. The NF mRNA expression significantly decreased while the level of m-calpain and μ-calpain significantly increased in two ACR-treated rats groups regardless of the ACR dose. The light NF （NF-L） protein expression was significantly correlated with NF-L mRNA expression. Combined with previous data, the concentrations of three NF subunits were negatively correlated with the calpain levels. These findings suggest that NF-L mRNA and calpain mediated the reduction in NF of AER neuropathy.The purpose of this study was to study the role of neurofilament （NF） mRNA and calpain in NF reduction of acrylamide （ACR） neuropathy. Male Wistar adult rats were injected i.p. every other day with AER （20 mg/kg.bW or 40 mg/kg.bW） for 8 weeks. NF mRNA expression was detected using RT-PCR and the calpain concentration was determined using western blot analysis. The NF mRNA expression significantly decreased while the level of m-calpain and μ-calpain significantly increased in two ACR-treated rats groups regardless of the ACR dose. The light NF （NF-L） protein expression was significantly correlated with NF-L mRNA expression. Combined with previous data, the concentrations of three NF subunits were negatively correlated with the calpain levels. These findings suggest that NF-L mRNA and calpain mediated the reduction in NF of AER neuropathy.

关 键 词：neuropathy acrylamide Calpain calpain injected nerves abundant neuronal splay supernatant 

分 类 号：R114[医药卫生—卫生毒理学]