Multicenter Evaluation of the Molecular Line Probe Assay for Multidrug Resistant Mycobacterium Tuberculosis Detection in China  

作　　者：LI Qiang DONG Hai Yan PANG Yu XIA Hui OU Xi Chao ZHANG Zhi Ying LI Jun Chen ZHANG Jian Kang HUAN Shi Tong CHIN Daniel P KAM Kai Man ZHAO Yan Lin 

机构地区：[1]National Tuberculosis Reference Laboratory,Chinese Center for Disease Control and Prevention [2]PATH Beijing Office [3]Gates Foundation Beijing Office [4]Chinese University of Hong Kong

出　　处：《Biomedical and Environmental Sciences》2015年第6期464-467,共4页生物医学与环境科学（英文版）

基　　金：supported by Bill&Melinda Gates Foundation Tuberculosis Prevention and Control Project(2009-04-01)

摘　　要：In order to evaluate the performance of a molecular Hain line probe assay （Hain LPA） for rapid detection of rifampicin and isoniazid resistance of Mycobocterium tuberculosis in China, 1612 smear positive patients were consecutively enrolled in this study. Smear positive sputum specimens were collected for Hain LPA and conventional drug susceptibility testing （DST）. The sensitivity and specificity of Hain LPA were analyzed by using conventional DST as golden reference. The sensitivity, specificity, positive predictive value {PPV） and negative predictive value （NPV） for rifampicin resistance detection were 88.33%, 97.66%, 81.54%, and 98.62%, respectively. The sensitivity, specificity, PPV and NPV for isoniazid resistance detection were 80.25%, 98.07%, 87.25%, and 96.78%, respectively. These findings suggested that Hain LPA can be an effective method worthy of broader use in China.In order to evaluate the performance of a molecular Hain line probe assay （Hain LPA） for rapid detection of rifampicin and isoniazid resistance of Mycobocterium tuberculosis in China, 1612 smear positive patients were consecutively enrolled in this study. Smear positive sputum specimens were collected for Hain LPA and conventional drug susceptibility testing （DST）. The sensitivity and specificity of Hain LPA were analyzed by using conventional DST as golden reference. The sensitivity, specificity, positive predictive value {PPV） and negative predictive value （NPV） for rifampicin resistance detection were 88.33%, 97.66%, 81.54%, and 98.62%, respectively. The sensitivity, specificity, PPV and NPV for isoniazid resistance detection were 80.25%, 98.07%, 87.25%, and 96.78%, respectively. These findings suggested that Hain LPA can be an effective method worthy of broader use in China.

关 键 词：Mycobacterium Probe tuberculosis detecting sequenced phenotypic likely inconsistent specimen elsewhere 

分 类 号：R446.5[医药卫生—诊断学]