HPLC法检测人胆汁中利奈唑胺浓度  

Determination of linezolid in human bile by HPLC

作　　者：黄成坷[1] 周伶俐[2] 孙未[1] 王哲[1] 王增寿[1] 

机构地区：[1]温州医科大学附属第二医院药学部,温州325027 [2]温州医科大学附属第二医院病理科,温州325027

出　　处：《中国临床药学杂志》2015年第2期103-106,共4页Chinese Journal of Clinical Pharmacy

基　　金：浙江省药学会医院药学专项科研资助项目(编号2014ZYY41)

摘　　要：目的建立检测人胆汁中利奈唑胺浓度的HPLC法。方法以氯霉素为内标，色谱柱为Eclipse XDB C18（250mm×4．6mm，5μm），流动相为甲醇-水（45：55），流速1．0mL·min^1，柱温30％，检测波长254nm。结果利奈唑胺在质量浓度0．50-100mg·L^-1内线性关系良好（r=0．9998），定量下限为0．50mg·L^-1；低、中、高浓度的日间、日内精密度RSD均〈10％，相对回收率分别为98．62％、101．32％、100．64％，提取回收率〉75％。结论本方法操作简便，结果准确，适用于胆汁中利奈唑胺的检测。AIM To establish an HPLC method for the determination of linezolid in human bile. METHODS Chloramphenicol was used as the internal standard. The analytical column was packed with Eclipse XDB-C18（250mm×4.6mm,5μm） the mobile phase was methanol-water（45：55）, the flow rate was 1.0 mL·min^-1, the temperature was 30% and the detection wavelength was 254 nm. RESULTS The liner range of linezolid in human bile was 0.50 - 100 mg·L^-1（ r = 0.999 8）. The limit of quantitation was 0.50 mg·L^-1. The intra-day and inter-day RSD for assaying the bile sample containing three concentration of linezolid were both lower than 10%, the relative recovery was 98.62%, 101.32% and 100.64% ,and the absolute recovery was more than 75%. CONCLUSION The HPLC method to determine linezolid in human bile is simple, high selectivity and sensitivity.

关 键 词：利奈唑胺 胆汁 高效液相色谱法 

分 类 号：R96[医药卫生—药理学] O657.72[医药卫生—药学]