模拟空间环境X射线和微重力对大鼠骨丢失的影响  

Effect of X-ray radiation and microgravity on bone loss

作　　者：杨晓勇[1,2] 唐辉[2] 徐永清[2] 蔡志军[3] 赵庆凯[1,2] 汤逊[1,2] 

机构地区：[1]第三军医大学研究生管理大队,重庆400038 [2]成都军区昆明总院附属骨科医院全军创伤骨科研究所,昆明650032 [3]昆明医科大学昆明总院临床分院,昆明650032

出　　处：《第三军医大学学报》2015年第12期1232-1236,共5页Journal of Third Military Medical University

基　　金：云南省科技厅支持项目(2010BC006)~~

摘　　要：目的探讨辐照和微重力对于骨代谢是否存在交互作用。方法 2月龄雄性Wistar大鼠分为正常对照组、X射线辐照组(X-ray组)、尾吊模型组(HLS组)及尾吊结合X射线辐照组(HLS+Xray组)。分别对HLS组和HLS+X-ray组进行4 Gy X射线全身辐照,对HLS+X-ray组进行尾吊实验来模拟空间环境下辐射和微重力的条件,检测骨密度、骨小梁的数目、骨小梁的分离程度、骨小梁的厚度、骨体积分数、结构模型指数等参数并检测了骨碱性磷酸酶(ALP)、酒石酸酸性磷酸酶(TRAP)、骨钙素(OCN)的及骨调节基因Rankl、Runx2、Nfatcl的表达变化,研究两种因素所引起的骨丢失是否有相应的交互作用。结果尾吊和X射线辐照在骨丢失方面引起了显著的变化,同正常对照组相比,HLS组和X-ray组的骨密度、骨小梁的数目、骨小梁的厚度、骨体积分数均表现为降低趋势(P<0.05),HE染色显示HLS组、X-ray组和HLS+X-ray组中都出现骨小梁数量减少等特征,说明各个模型组都存在骨丢失现象,以HLS+X-ray组最为明显(P<0.05)。Rankl、Nfatcl以及酒石酸酸性磷酸酶的表达在HLS组、Xray组及HLS+X-ray组均上升(P<0.05)。碱性怜酸酶和骨钙素的表达在HLS组、X-ray组及HLS+Xray组均有所下降(P<0.05)。而作为成骨细胞特异性表达的Runx2基因在HLS组和X-ray组却没有任何改变,在HLS+X-rays组发生了显著的下调(P<0.05)。结论尾吊和X射线双因素能够引起骨密度更大的丢失,两者存在交互作用,破坏程度会更加严重。Objective To determine the effect of X-ray radiation and microgravity on bone metabolism. Methods Male Wistar rats were divided into a control group,an X-ray radiation group（ X-ray group）,a hindlimb suspension group（ HLS group）,and a hindlimb suspension and X-ray radiation group（ HLS ＋ X-ray group）. The X-ray radiation dose was 4 Gy,and the hindlimb suspension was adopted to simulate microgravity environment. The bone mineral density,trabecular number,trabecular separation,trabecular thickness,bone volume / trabecular volume,and structural model index were detected,and the changes of bone alkaline phosphatase（ ALP）,tartrate-resistant acid phosphatase（ TRAP）,osteocalcin（ OCN）,and bone regulatory genes（ Rankl,Runx2 and Nfatcl） were analyzed. Results The hindlimb suspension and X-ray radiation caused significant reduction（ P 〈 0. 05） in bone mineral density,trabecular number,trabecular thickness,and bone volume / trabecular volume in the HLS group,X-ray group and HLS ＋X-ray group. The bone loss was the most severe in the HLS ＋ X-ray group（ P 〈 0. 05）. The expression of Rankl,Nfatcl and TRAP were significantly increased（ P 〈 0. 05） in the HLS group,X-ray group and HLS ＋X-ray group,while the expression of ALP and OCN was significantly decreased（ P 〈 0. 05）. Moreover,Runx2 was not changed in the HLS group and X-ray group,but was down-regulated in the HLS ＋ X-ray group（ P 〈 0. 05）. Conclusion The combination of hindlimb suspension and X-ray radiation can more severe bone loss than alone.

关 键 词：骨丢失 模拟微重力 X射线辐照 骨小梁参数 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学] R852.22[医药卫生—基础医学]