慢性运动性中枢疲劳大鼠海马5-羟色胺及血清氨基酸的相关研究  

Association of Serotonin with Tryptophan and Branched Chain Amino Acids in Rat's Hippocampus with Central Fatigue after Exercise

作　　者：刘占东[1] 吴岩珏 王得新[3] 刘天会[4] 李任[5] 谢敏豪[6] 薛启蓂[3] 

机构地区：[1]首都医科大学附属北京友谊医院医保中心神经内科,100050 [2]美国疾病控制与预防中心 [3]首都医科大学附属北京友谊医院神经内科,100050 [4]首都医科大学附属北京友谊医院实验中心,100050 [5]首都医科大学附属北京友谊医院药理研究室,100050 [6]北京体育大学,100084

出　　处：《中国临床神经科学》2015年第3期274-279,共6页Chinese Journal of Clinical Neurosciences

基　　金：国家自然科学基金(编号:SN 81041019);国家科技支撑项目(编号:SN 2012BAK21B02);北京市保健科研课题(编号:SN京13-03)资助

摘　　要：目的探讨慢性运动性中枢疲劳大鼠海马5-羟色胺(5-HT)与血清中色氨酸和支链氨基酸的含量变化特点。方法建立慢性运动性中枢疲劳大鼠模型,采用高效液相色谱-质谱方法测定模型大鼠的血清色氨酸和支链氨基酸水平,以及海马组织的5-HT含量。结果慢性运动性中枢疲劳大鼠血清色氨酸水平明显降低;3种支链氨基酸的测定水平虽差异无统计学意义,但其含量与色氨酸的比值均出现明显升高。结论慢性运动性中枢疲劳大鼠血清色氨酸水平的降低有利于支链氨基酸进入中枢,该机制可能影响中枢5-HT含量的变化。Aim To analyze the relationship of hippocampus serotonin with alternations of serum tryptophan and branched chain amino acids （BCAA） on the basis of improved Rats’ Chronic Exercise-induced Central Fatigue Model.Methods① Rats’ Chronic Exercise-induced Central Fatigue Model was established.② The method of HPLC-MC to measure the level of tryptophan and BCAA in rat’s serum was adopted and the relationship between them was analyzed.③ The method of HPLC-ECD to measure the content of 5-HT in rat hippocampus was used.Results It was easily found that there was a signiifcant decrease of serum tryptophan in rats with central fatigue after running （P〈0.01）, compared with the control＆amp;nbsp;group, while BCAA （serum leucine, isoleucine and valine）, the rival competitor of tryptophan when entered into the central nervous system, were not signiifcantly changed. Ratios of leucine, isoleucine and valine to tryptophan were also signiifcantly increased （P〈0.01）, and the ratio of sum of BCAA to tryptophan as well. Conclusion Rats’ level of 5-HT had a sharp increase with both chronic central fatigue after exercise and after one-time exhaustive exercise. But there was some difference in regulation mechanism between them. The fall of serum tryptophan and the rise of the ratio of BCAA/TRP were favorable for BCAA to enter the central nerve. And at the same time, the measurement of tryptophan in serum was likely to become the biochemical indicators of the extreme fatigue of organism.

关 键 词：中枢疲劳 色氨酸 支链氨基酸 5-羟色胺 

分 类 号：Q189[生物学—神经生物学] R338[生物学—普通生物学]