不同类型肝炎妊娠期妇女中HBV DNA与PreS1Ag表达关系的研究  

Correlation Between Hepatitis B Virus Genome Viral Load and PreS1Ag in Pregnancy Women

作　　者：曹登成 杨淑哲 

机构地区：[1]成都市妇女儿童中心医院检验科,四川成都610091

出　　处：《标记免疫分析与临床》2015年第7期630-632,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨妊娠期妇女血清中乙肝病毒(HBV DNA)与前S1抗原(Pre S1Ag)表达的相关性。方法以2013年1月至2014年1月在本院进行乙肝病毒(HBV DNA)检测的558例妊娠期妇女为研究对象,采用实时荧光定量PCR技术检测血清中HBV DNA表达载量,酶联免疫法检测血清中Pre S1Ag的表达。结果 221例HBV DNA阳性标本中,Pre S1Ag的阳性率为88.6%(196/221),阴性率为11.4%(25/221);337例HBV DNA阴性标本中,Pre S1Ag的阳性率为56.9%(192/337),阴性率为43.1%(145/337),其差异有统计学意义(P<0.05);在HBV DNA阳性的标本中,随着病毒载量递增,Pre S1Ag阳性率并未升高(P>0.05)。结论 HBV DNA的表达与Pre S1Ag阳性率具有一定的相关性,但病毒载量与Pre S1Ag阳性率不相关,HBs Ag/HBe Ag/HBc三项阳性(俗称"大三阳")模式中HBV DNA与Pre S1Ag表达具有相关性。HBs Ag/抗HBe/抗HBc三项阳性(俗称"小三阳")模式中HBV DNA与Pre S1Ag表达无相关性。提示对于小三阳的妊娠期女性HBV DNA监测更具临床意义。Objective To investigate correlation between hepatitis B virus genome viral load and PreS1Ag in pregnancy women. Methods 558 cases of pregnancy women were involved in this study, and the levels of HBV DNA were detected by PCR technique and PreS1Ag were detected by enzyme linked immunoassay. Results The PreS1Ag positive rate was 88.6% in 221 HBV DNA positive cases and the PreS1Ag positive rate was 56.9% in 337 HBV DNA negative cases（P 〈0.05）. The positive rate of PreS1Ag was not increased with the HBV DNA increasing in the HBV DNA positive samples. There was correlation between positive rate of HBV DNA and PreS1Ag, but no correlation between hepatitis B virus genome viral load and PreS1Ag. There is correlation between positive rate of HBV DNA and PreS1 Ag in the samples which the model was HBsAg, HBeAg and HBc positive. There was no correlation between positive rate of HBV DNA and PreS1Ag in the samples which the model was HBsAg, anti-HBe and anti-HBc positive. Conclusion The results in this study suggest that HBV DNA test are more important in the pregnancy women who have HBsAg, anti-HBe and anti-HBc positive.

关 键 词：乙肝病毒DNA 前S1抗原 相关性 妊娠期 

分 类 号：R714.251[医药卫生—妇产科学]