鼻病毒非结构蛋白2B诱导细胞发生内质网应激  

Endoplasmic reticulum stress induced by human rhinovirus 16 2B protein

作　　者：迟苗苗 宋娟[1] 宋芹芹[1] 于洁[1] 罗小暖[1] 张璐[1] 王欣玲[1] 韩俊[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《微生物与感染》2015年第3期154-158,共5页Journal of Microbes and Infections

基　　金：国家自然科学基金(31371397);传染病预防控制国家重点实验室发展基金(2011SKLID104)

摘　　要：为探讨鼻病毒非结构蛋白2B诱导内质网应激和细胞凋亡的机制,本研究构建了鼻病毒非结构蛋白2B的真核表达载体p2B-GFP,通过转染BHK-21细胞检测相关标志蛋白的变化情况。结果显示,非结构蛋白2B定位表达于BHK-21细胞内质网,诱导内质网应激标志蛋白Grp78、CHOP的表达增加,并使活化转录因子6(ATF6)的转录活性增加,还诱导BHK-21细胞发生核浓缩而凋亡,使凋亡标志蛋白PARP发生降解而减少。结果提示,鼻病毒非结构蛋白2B可诱导细胞发生内质网应激,并经该途径诱导细胞凋亡。To investigate whether the non‐structural protein 2B of rhinovirus 16 induces endoplasmic reticulum stress （ERS） and apoptosis ,BHK‐21 cells were transfected with a plasmid p2B‐GFP expressing rhinovirus 16 2B protein .Biomarkers of ERS and apoptosis were detected respectively .The results showed that 2B protein was localized to the ER apparatus .2B protein increased the expressions of ERS marker proteins Grp 78 and CHOP and the transcriptional activity of activating transcription factor 6 （ATF6） .We also found 2B protein induced nuclear condensation associated with apoptosis and degradation of marker protein PARP in BHK‐21 cells .In conclusion ,the rhinovirus 16 2B protein induces both ERS and apoptosis .

关 键 词：鼻病毒 非结构蛋白2B 内质网应激 凋亡 

分 类 号：R373[医药卫生—病原生物学]