分子群体遗传学方法处理鲤形态学数据的适用性  

Applicability of molecular population genetics methods in analyzing common carp morphological data

作　　者：林明雪[1] 王剑 王军[1] 李康乐[1] 古珍珍 项松平 徐志彬 贾延民 鲁国延 王成辉[1] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [2]浙江龙泉省级瓯江彩鲤良种场,浙江龙泉323700 [3]陕西延安市水产工作站,陕西延安716000

出　　处：《水产学报》2015年第6期769-778,共10页Journal of Fisheries of China

基　　金：国家自然科学基金(30972250;31372521;31372521);上海市科委项目(123919N1100)

摘　　要：在鱼类形态学性状的数据处理分析中,主要利用数理统计分析软件进行形态学数据的聚类分析、主成分分析和判别分析,而随着现代分子生物学技术的发展,一系列分子群体遗传学数据分析方法和处理软件应运而生。为研究分子群体遗传学方法对鱼类形态学数据分析的适用性,实验以兴国红鲤、玻璃红鲤、荷包红鲤完全双列杂交产生的9个群体形态学数据为对象,比较了传统形态学方法与分子群体遗传学方法对鱼类形态学性状的分析效果。结果发现,两类方法的聚类分析结果一致;主成分分析的结果表明,传统形态学分析方法能反映个体间的形态差异,而分子群体遗传学方法则能更好地反映群体间的形态差异;判别分析结果显示,两类方法的综合判别率相当;利用STRUCTURE软件进行的遗传聚类分析结果也发现,两类方法所得的结果一致。研究表明,应用传统形态学方法与分子群体遗传学方法分析鲤形态学性状是一致的,分子群体遗传学方法处理鲤科鱼类形态学数据是可行的。The traditional morphological methods implemented in mathematical statistic software, including clustering analysis,principal component analysis and discriminant analysis,have been widely used in dealing with morphological data in fishes. However, a series of molecular genetics software have also been developed coupling with development of modern molecular biology techniques. In this paper, in order to study the applicability of the molecular population genetics methods in analyzing fish morphological data, the morphological traits of nine mating combinations from a complete diallel cross of Xingguo red common carp （C. carpio var. singuonensis） , Glass red common carp （C. carpio var. wananensis）and Purse red common carp（ C. carpio var. wuyuanensis）, were used to conduct comparative analysis of traditional morphological methods and molecular population genetics methods. The results showed that the same profiles of clustering analysis were found from the two kinds of methods. The principal component analysis indicated that the traditional morphological methods can better reflect the morphological variations among individuals, while the molecular population genetics methods can better reflect the morphological differences among populations. The discriminant analysis showed the similar discriminant accuracy of the two kinds of methods. Furthermore, the genetic clustering analysis results using software STRUCTURE illustrated the consistency of the two kinds of methods. Consequently, the study produced the same results from the traditional morphological methods and molecular population genetics methods in dealing with morphological data of red common carp. It is feasible to analyze morphological data of common carp using molecular population genetics methods.

关 键 词：鲤 形态性状 多元分析 分子遗传学 

分 类 号：Q348[生物学—遗传学] S917.4[农业科学—水产科学]