天麻钩藤饮抗大鼠视神经夹伤模型视网膜神经节细胞凋亡作用的实验研究  

Effect of Tianma Gouteng Decoction on retinal ganglion cells apoptosis in rat optic nerve crush model

作　　者：李玉洁[1] 张海娟[1] 王海燕[1] 马科[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科研究所眼科学与视觉科学北京市重点实验室国家眼科创新药物临床评估中心,100005

出　　处：《眼科》2015年第3期188-192,共5页Ophthalmology in China

基　　金：国家自然科学基金(81102617)

摘　　要：目的观察天麻钩藤饮对视神经夹伤模型大鼠视网膜神经上皮层厚度和视网膜神经节细胞(RGC)凋亡的影响。设计实验研究。研究对象 SPF级雄性Wistar大鼠48只。方法将大鼠随机分6组,每组8只,分别为正常对照组,阴性对照组,天麻钩藤饮低剂量组(0.6 g/ml)、中剂量组(1.2 g/ml)、高剂量组(2.4 g/ml)和银杏叶片阳性对照组(1.2 mg/ml),除正常对照组大鼠不做处理,其他组大鼠右眼均建立视神经夹伤模型,正常对照组和阴性对照组给予纯净水灌胃。于给药30天处死动物取眼球,做石蜡切片HE染色,观察各组视网膜神经上皮层厚度,TUNEL法检测RGC的凋亡程度。主要指标 HE染色视网膜神经上皮厚度及RGC凋亡数量。结果阴性对照组(171.04±13.86μm)比正常组(208.98±8.46μm)视网膜神经上皮厚度显著减少(P=0.000)。而天麻钩藤饮中、高剂量组大鼠视网膜厚度(分别为187.68±11.16μm和189.22±9.54μm)比阴性对照组显著增加(P=0.043,0.001),且中、高剂量组与阳性对照组(191.35±9.03μm)之间无显著差异(P=0.052,0.670);TUNEL凋亡检测发现,阴性对照组凋亡细胞数(9.09±2.24个/高倍视野)比正常组(0.59±0.61个/高倍视野)明显增加(P=0.000),中剂量组、高剂量组和阳性对照组RGC的凋亡(分别为7.00±1.88,5.22±2.05,5.03±2.03个/高倍视野)均比阴性对照组显著减少(P=0.024,0.000,0.000)。结论天麻钩藤饮对大鼠视神经夹伤模型具有一定抗RGC凋亡的作用,随药物浓度的升高,抗凋亡作用更显著。Objective To observe the effect of Tianma Gouteng Decoction on the retinal neuroepithelial thickness and retinal ganglion cells （RGCs） apoptosis in the optic nerve crush model rats. Design Experimental study. Participants Forty-eight male Wistar rats. Methods Rats were divided randomly into six groups： normal control, model, Tianma Gouteng Decoction treatment groups （con- centrations were 0,6 g/ml, 1.2 g/ml, 2.4 g/ml） and Ginkgo biloba tablets positive control group （concentrations was 1.2 mg/ml）, treat- ed for 30 days. Nothing was done in the normal control group. The optic nerve of right eye in the other groups was crushed with 40 g microclip 2 mm behind the globe for 60 seconds, the left eye was uncrushed. Observing the retinal neuroepithelial thickness under HE stained paraffin sections and the RGCs apoptosis in retina was assayed by TUNEL staining. Main Outcome Measures The retinal neuroepithelial thickness, RGC apptosis counts. Results The retinal neuroepithelial thickness was significantly decreased in the model group （ 171.04 ± 13.86 μm ） than in the normal controls （ 208.98 ± 8.46 μm ） （P=0.000）, while the retinal neuroepithelial thickness was significantly increased in the medium and high dose Tianma Gouteng Decoction groups （ 187.68 ± 11.16 txm and 189.22 ± 9.54 μm ） than in the model group （P=0.043, 0.001）; There was no significant difference between the medium dose group, high dose group and positive control group （ 191.35 ± 9.03 μm ） （P=0.052, 0.670）; The positive TUNEL cells in model group （ 9.09 ± 2.24 cells/HP ） increased obviously, which was significantly different from the normal control group （ 0.59 ± 0.61 cells/HP ） （P=-0.000）. The apopto- sis cells were significantly decreased in the medium dose group, high dose group and positive control group （ 7.00 ± 1.88, 5.22 ± 2.05, 5.03 ± 2.03 cells/HE reslaectivelv ） than the neRative control Rrouo （P=0.024, 0.000, 0.000）. Conclusions Tianma Gouten Decoctionhas certain effect

关 键 词：天麻钩藤饮 视神经夹伤 视网膜神经上皮层 视网膜神经节细胞 凋亡 

分 类 号：R774.1[医药卫生—眼科] R-332[医药卫生—临床医学]