肥大细胞脱颗粒的神经免疫调控机制研究  

An in vitro study on substance P-stimulated neuro-immune mechanism of mast cell degranulation

作　　者：李秋婷[1] 赵长青[1] 

机构地区：[1]山西医科大学第二医院耳鼻咽喉头颈外科,太原030001

出　　处：《临床耳鼻咽喉头颈外科杂志》2015年第12期1118-1120,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金(No:81271059);山西省回国留学人员重点科研资助项目(No:2014-重点3);山西省科技攻关项目(No:20130313022-1)

摘　　要：目的:初步探讨神经肽P物质刺激肥大细胞脱颗粒的神经调控机制。方法:分A、B两组进行体外诱导小鼠骨髓肥大细胞。A组培养液中加入SCF、IL-3和IL-4,B组培养液中加入SCF、IL-3诱导分化,培养4周后收集细胞备用。Western Blot分析各组骨髓肥大细胞FcεRⅠα和NK-1R表达情况。两组分别加入不同浓度神经肽P物质孵育30min,检测上清液和细胞内组胺含量,计算组胺释放率。结果:A组FcεRⅠα和NK-1R的表达均较B组高(P<0.05);P物质可促发A、B两组肥大细胞脱颗粒,且同一浓度P物质刺激时,A组肥大细胞脱颗粒程度高于B组(P<0.05)。结论:P物质介导的肥大细胞脱颗粒的神经调控机制可能存在由其特异受体NK-1R介导的非免疫性及FcεRⅠα介导的免疫性双途径,为深入研究鼻黏膜高反应性鼻病的发病机制以及临床治疗策略提供了新思路。Objective：The goal of this study was to study the mechanism of substance P（SP）-mediated the neural control of mast cell（MC）degranulation.Method：Bone marrow mast cells from mice were cultured with stem cell factor（SCF）,IL-3and IL-4（group A）and SCF,IL-3（group B）for four weeks.Then the cells were harvested and reserved for studies.Western Blot hybridization technique was used to detect the expression of FcεRⅠαand NK-1Ron MCs from the two groups.Then such cells were activated with SP（0,0.01,0.10,1.00,10.00μg/ml,respectively）for 30 min.The histamine released into the supernatant and stored in the protoplasm was quantified by enzyme linked immunosorbent assay（ELISA）.And the percentage of histamine release was calculated as a percent of total histamine content.Result：The expressions of FcεRⅠαand NK-1Ron these mast cells in group A were statistically higher than in group B（P〈0.05）.The MCs from two groups can be actived when stimulated by SP,but the level of MC degranulation in group A was higher than group B（P〈0.05）.Conclusion：Neuropeptide may stimulate MC degranulation through immunological and non-immunological pathways.In summary,the current study provides us with better understanding of the mechanism of neuropeptide-controlled MC deranulation,and this should be helpful for the further research involved in the mechanism and treatmemt of airway hyper-reactivity.

关 键 词：肥大细胞 神经肽 脱颗粒 

分 类 号：R765[医药卫生—耳鼻咽喉科]