养血补肾方对豚鼠形觉剥夺性近视眼巩膜细胞凋亡的影响  

Effect of nourishing blood and tonifying kidney decoction on form deprivation myopia in guinea pig sclera cell apoptosis

作　　者：侯静梅[1] 吴宁玲[2] 亢泽峰[2] 

机构地区：[1]青海大学,西宁810001 [2]中国中医科学院眼科医院,北京100040

出　　处：《中国中医眼科杂志》2015年第2期77-80,共4页China Journal of Chinese Ophthalmology

基　　金：国家青年科学自然基金项目(81303009)

摘　　要：目的观察养血补肾方对形觉剥夺性近视眼巩膜组织的作用。方法取2周龄健康豚鼠,以半透明眼罩单眼遮盖60d制备豚鼠形觉剥夺性近视模型,将造模成功豚鼠随机分为治疗组和对照组,治疗组灌胃法给予养血补肾方水煎液(生药量12.7g/kg),共15 d,每天1次,对照组不进行干预。测量造模前后双眼屈光度和眼轴,HE染色法观察巩膜组织形态学变化,流式细胞术检测巩膜细胞的凋亡。结果造模眼的眼轴增长,呈近视性屈光改变,同期非造模眼为远视性屈光状态,眼轴明显短于模型眼(P<0.05)。光镜检查显示,治疗组模型眼的后极部巩膜纤维存在一定的病理学改变,但明显好于对照组的模型眼。各组巩膜细胞的凋亡率分别为:治疗组模型眼(A)(49.81±6.59)%,对照组模型眼(B)(74.21±3.63)%,对照组非造模眼(C)(24.46±3.51)%,治疗组模型眼的巩膜细胞凋亡率居中,高于非造模眼并低于对照组模型眼(F=161.580,P<0.001;两两比较,A与B,P<0.001,A与C,P<0.001,B与C,P<0.001)。结论养血补肾方能够明显改善豚鼠形觉剥夺性近视眼巩膜组织的形态,其保护作用可能与降低巩膜细胞的凋亡有关。OBJECTIVE To observe the effects of nourishing blood and tonifying kidney decoction on form deprivation myopia sclera tissue. METHODS The models of form deprivation myopia were made on 2 weeks healthy guinea pigs through translucent goggles monocular covered 60 d. Successfully built guinea pigs were randomly divid-ed into control group and dosing group. The guinea pigs of dosing group were lavaged nourishing blood and tonifying kidney decoction once a day for 15 d. The control group received no intervention. The binocular diopter and axial were measured before and after the modeling. The morphological changes of scleral tissue were observed by HE staining method, and the apoptosis of scleral fibroblasts was detected by flow cytometry. RESULTS The model eye ’s axial length was longer with myopic refractive change. Non-modal eyes were hyperopic refractive status, and the axi-al length was significantly shorter than that of the model group （P＆lt;0.05）. Light microscopy examination showed that there was certain pathology change in posterior sclera fiber of the eye model of dosing group, but much better than that of control group. The rate of apoptosis of sclera fibroblasts of each group：The model eye of dosing group：（A） （49.81±6.59）%, The model eye of control group （B） （74.21±3.63）%,non-model eye of the control group （C） （24.46±3.51）%,the rate of apoptosis of the model eye of the dosing group was in the middle which was higher than that of non-model eye but lower than that of the control group, F=161.580,P＆lt;0.001;Paired comparison was signifi-cant, A and B, P＆lt;0.001,A and C,P＆lt;0.001,B and C,P＆lt;0.001. CONCLUSIONS Nourishing blood and tonifying kidney could significantly improve the form deprivation myopia in the guinea pig scleral tissue morphology, and its protective effect may be related to the decrease of scle-ral fibroblasts apoptosis.

关 键 词：养血补肾方 形觉剥夺性近视 巩膜 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R774.1[医药卫生—中医学]