Species and endosymbiont diversity of Bemisia tabaci （Homoptera： Aleyrodidae） on vegetable crops in Senegal  

作　　者：Delatte Helene Baudin Remy Becker Nathalie Girard Anne-Laure Ramatoulaye Sidebe Traore Lett Jean-Michel Reynaud Bernard 

机构地区：[1]CIRAD, UMR PVBMT, F-97410 Saint-Pierre, La Reunion, France [2]Museum National d'Histoire Naturelle, Institut de Systematique, Evolution, Biodiversite, ISYEB, UMR 7205 CNRS, UPMC, EPHE, 75005 Paris, France [3]TECHNISEM, Zone d'activite Anjou Actiparc de Jumelles, 49160 Longue-Jumelles, France

出　　处：《Insect Science》2015年第3期386-398,共13页昆虫科学（英文版）

摘　　要：Bemisia tabaci-transmitted geminiviruses are one of the major threats on cassava and vegetable crops in Africa. However, to date, few studies are available on the diversity orB. tabaci and their associated endosymbionts in Africa. More than 28 species have been described in the complex ofB. tabaci cryptic species; among them, 2 are invasive pests worldwide： MED and MEAM1. In order to assess the species diversity orB. tabaci in vegetable crops in Senegal, several samplings in different localities, hosts and seasons were collected and analyzed with nuclear （microsatellite） and mitochondrial （COI） markers. The bacterial endosymbiont community was also studied for each sample. Two species were detected： MED Q1 and MEAM1 B. Patterns of MED Q1 （dominance on most of the samples and sites, highest nuclear and mitochondrial diversity and broader secondary endosymbiont community： Hamiltonella, Cardinium, Wolbachia and Rickettsia）, point toward a predominant resident begomovirus vector group for MED Q 1 on market gardening crops. Furthermore, the lower prevalence of the second species MEAM1 B, its lower nuclear and mitochondrial diversity and a narrower secondary endosymbiont community （Hamiltonella/Rickettsia）, indicate that this genetic group is exotic and results from a recent invasion in this area.Bemisia tabaci-transmitted geminiviruses are one of the major threats on cassava and vegetable crops in Africa. However, to date, few studies are available on the diversity orB. tabaci and their associated endosymbionts in Africa. More than 28 species have been described in the complex ofB. tabaci cryptic species; among them, 2 are invasive pests worldwide： MED and MEAM1. In order to assess the species diversity orB. tabaci in vegetable crops in Senegal, several samplings in different localities, hosts and seasons were collected and analyzed with nuclear （microsatellite） and mitochondrial （COI） markers. The bacterial endosymbiont community was also studied for each sample. Two species were detected： MED Q1 and MEAM1 B. Patterns of MED Q1 （dominance on most of the samples and sites, highest nuclear and mitochondrial diversity and broader secondary endosymbiont community： Hamiltonella, Cardinium, Wolbachia and Rickettsia）, point toward a predominant resident begomovirus vector group for MED Q 1 on market gardening crops. Furthermore, the lower prevalence of the second species MEAM1 B, its lower nuclear and mitochondrial diversity and a narrower secondary endosymbiont community （Hamiltonella/Rickettsia）, indicate that this genetic group is exotic and results from a recent invasion in this area.

关 键 词：begomovirus vectors endosymbionts diversity MED and MEAM 1 species microsatellites mtCOI phylogeny whitefly 

分 类 号：S432.41[农业科学—植物病理学] S718.54[农业科学—农业昆虫与害虫防治]