Identification and localization of two sensory neuron membrane proteins from Spodoptera fitura （Lepidoptera： Noctuidae）  

作　　者：Jin Zhang Yang Liu William B. Walker Shuang-Lin Dong Gui-Rong Wang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing [2]Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China [3]Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Chemical Ecology Research Group, Alnarp, Sweden

出　　处：《Insect Science》2015年第3期399-408,共10页昆虫科学（英文版）

基　　金：We would like to thank Li-Yan Yang for insect rearing, Shu-Wei Yan and Meng-Bo Guo for technical assistance in the qRT-PCR and in situ hybridization. This work was supported by the National Basic Research Program of China （Grant No. 2012CB114104）, the National Natural Science Foundation of China （Grant No. 31230062 and 31071752） and Beijing Natural Science Foundation （6132028）.

摘　　要：Sensory neuron membrane proteins （SNMPs）, which are located on the dendritic membrane of olfactory sensory neurons （OSNs）, are proposed to be associated with odor reception in insects. Recent studies have demonstrated that SNMP1 is essential for electrophysiological responses of OSNs to the sex pheromone, cis-vaccenyl acetate （cVA） in Drosophila melanogaster. To investigate the function ofLepidoptera SNMPs, we cloned two SNMP genes, SlituSNMP1 and SltiuSNMP2, from Spodoptera litura （Lepidoptera： Noctuidae）. Sequence alignment and phylogenetic analysis showed that both genes bear the general characteristics of SNMPs, including six conserved cysteine residues and two transmembrane domains. Further expression profile experiments showed that SIituSNMP1 is mainly expressed in the antenna, while SlituSNMP2 is broadly expressed in various tissues. By in situ hybridization experiments, it was found that SlituSNMP1 expressing cells are surrounded by the SlituSNMP2 expressing cells in the pheromone sensitive sensilla, suggesting different fimctions ＆the two SNMPs in insect olfaction.Sensory neuron membrane proteins （SNMPs）, which are located on the dendritic membrane of olfactory sensory neurons （OSNs）, are proposed to be associated with odor reception in insects. Recent studies have demonstrated that SNMP1 is essential for electrophysiological responses of OSNs to the sex pheromone, cis-vaccenyl acetate （cVA） in Drosophila melanogaster. To investigate the function ofLepidoptera SNMPs, we cloned two SNMP genes, SlituSNMP1 and SltiuSNMP2, from Spodoptera litura （Lepidoptera： Noctuidae）. Sequence alignment and phylogenetic analysis showed that both genes bear the general characteristics of SNMPs, including six conserved cysteine residues and two transmembrane domains. Further expression profile experiments showed that SIituSNMP1 is mainly expressed in the antenna, while SlituSNMP2 is broadly expressed in various tissues. By in situ hybridization experiments, it was found that SlituSNMP1 expressing cells are surrounded by the SlituSNMP2 expressing cells in the pheromone sensitive sensilla, suggesting different fimctions ＆the two SNMPs in insect olfaction.

关 键 词：In situ hybridization Spodoptera litura sensory neuron membrane proteins （SNMPs） 

分 类 号：Q954.671[生物学—动物学] S433.4[农业科学—农业昆虫与害虫防治]