嘌呤核苷及其衍生物的代谢工程  

Progress in Biosynthesis of Purine Nucleosides and Their Derivatives by Metabolic Engineering

作　　者：王永成[1,2,3] 陈涛[1,2,3] 石婷[1,2,3] 王智文[1,2,3] 赵学明[1,2,3] 

机构地区：[1]天津大学化工学院,天津300072 [2]系统生物工程教育部重点实验室,天津300072 [3]天津化学化工协同创新中心,天津300072

出　　处：《中国生物工程杂志》2015年第5期87-95,共9页China Biotechnology

基　　金：国家"973"计划(2011CBA00804;2012CB725203);国家自然科学基金(21206112;21390201;21176182);国家"863"计划(2012AA02A702;2012AA022103)资助项目

摘　　要：嘌呤核苷及其衍生物被广泛应用于食品和医药领域。利用诱变筛选技术可以获得嘌呤核苷类产品的工业生产菌株,但往往耗时,效率低,而且获得的某些高产菌株还存在不稳定的缺陷。菌株代谢调控与生理生化的研究为代谢工程优化嘌呤核苷类产品的合成提供了理论基础,利用代谢工程改造菌株合成嘌呤核苷也引起了研究人员的关注。系统地介绍了微生物嘌呤生物合成途径及其调控机制,综述了嘌呤核苷类产品及其衍生物的代谢工程研究进展,最后讨论了利用代谢工程改造菌株合成这些产品面临的问题及今后的研究方向。Purine nucleosides and their derivatives play an very important role in food and pharmaceutical industry. In recent years, they have gained further importance because of their beneficial effects, related to their antioxidant, neuroproteetive and immunomodulatory properties. However, It is time consuming and low efficiency to get high-performing strains through mutation screening techniques. Moreover, some of these strains have disadvantage of genetic instability. With the better understanding of regulation mechanism of microorganism, metabolic engineering has been widely applied to improve the production of purine nucleoside products. In this article, the purine biosynthetic pathway and regulation mechanism are discussed, and progress in metabolic engineering of purine nucleosides and their derivatives is also reviewed. Meanwhile the current problems and future research direction are proposed.

关 键 词：嘌呤核苷 嘌呤生物合成途径 调控机制 代谢工程 

分 类 号：Q815[生物学—生物工程]