辛伐他汀对人甲状腺未分化癌细胞凋亡的影响  

Effects of simvastatin on apoptosis of human anaplastic thyroid carcinoma cells

作　　者：朱辉[1] 朱丹[2] 李兴佳[3] 徐书杭[3] 茅晓东[3] 刘超[3] 蔡可英[2] 

机构地区：[1]徐州医学院,江苏省221004 [2]徐州医学院第二附属医院内分泌病科 [3]南京中医药大学附属中西医结合医院内分泌代谢病院区

出　　处：《江苏医药》2015年第12期1377-1380,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金青年科学基金项目(81200577)

摘　　要：目的探讨辛伐他汀对人甲状腺未分化癌(ATC)细胞株HTh-74生长抑制和凋亡的作用。方法 MTT法检测细胞生长率;结晶紫染色观察细胞克隆形成情况;膜联蛋白Ⅴ/碘化丙啶(AnnexinⅤ/PI)双染检测细胞凋亡率;Western blot检测凋亡相关蛋白半胱氨酸蛋白酶3(Caspase-3)、B细胞淋巴瘤-白血病2(Bcl-2)和Bcl相关X蛋白(Bax)的表达。结果辛伐他汀对HTh-74细胞有显著生长抑制作用,呈浓度和时间依赖性(P<0.05或P<0.01)。辛伐他汀处理后可抑制细胞克隆形成、使细胞凋亡率显著增高、Caspase-3剪切体和Bax蛋白表达量增加、Bcl-2蛋白表达量降低、Bax/Bcl-2比值增加,且均呈浓度依赖性(P<0.05或P<0.01)。结论辛伐他汀可诱导人ATC细胞株HTh-74凋亡,其可能机制是与Bcl-2表达下调、Bax表达上调以及Caspase-3活化有关。Objective To explore the effects and possible mechanism of simvastatin on proliferation and apoptosis of human anaplastic thyroid carcinoma HTh‐74 cells .Methods The MTT assay ,crystal violet staining ,Annexin Ⅴ‐PI double staining and Western blot were used to determine cell proliferation rate ,cell clone formation ,cell apoptosis rate and the expression of apoptosis‐related proteins Caspase‐3 ,Bcl‐2 and Bax ,respectively .Results The proliferation of HTh‐74 cells was significantly inhibited by simvastatin in the concentration‐and time‐dependent manners （ P〈0 .05 or P〈0 .01） .Simvastatin could significantly inhibit the colony formation of HTh‐74 cells ,increase cell apoptosis rate and the expressions of cleav‐Caspase‐3 and Bax ,decrease the expression of Bcl‐2 ,and increase the ratio of Bax/Bcl‐2 ,which were all in a concentration‐dependent manner （ P〈0 .05 or P〈0 .01） .Conclusion Simvastatin can induce the apoptosis of human anaplastic thyroid carcinoma HTh‐74 cells ,which may be related to the downregulation of Bcl‐2 expression ,upregulation of Bax expression ,and activation of Caspase‐3 .

关 键 词：辛伐他汀 甲状腺未分化癌 HTh-74细胞 

分 类 号：R581[医药卫生—内分泌]