白藜芦醇对人咽鳞癌FADU细胞放射的增敏作用及其对细胞周期的影响  

The Radiosensitizing Effect of Resveratrol on Hopypharyngeal Carcinoma Cell Line FADU and its Effect on the Cell Cycle

作　　者：邵渊[1] 权芳[1] 李宏慧[1] 姚小宝[1] 赵谦[1] 赵瑞敏[1] 

机构地区：[1]西安交通大学医学院第一附属医院耳鼻咽喉头颈外科,西安710061

出　　处：《中国中西医结合杂志》2015年第6期699-703,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30901946)

摘　　要：目的应用白藜芦醇作用于咽鳞癌细胞株FADU,观察其放疗增敏作用。方法体外培养咽鳞癌细胞株FADU,以细胞毒实验(cytotoxicity test,MTT)检测不同浓度白藜芦醇对细胞增殖抑制情况;用克隆形成法绘制细胞存活曲线,获得白藜芦醇的放射增敏比(sensitive enhancement ratio,SER)。以流式细胞术(flow cytometry,FCM)分析对照组、单纯照射组、白藜芦醇组和照射增敏组(白藜芦醇联合照射组)细胞周期变化和凋亡情况。结果 MTT结果显示白藜芦醇对FADU细胞生长抑制作用随药物浓度的增加而增强(P<0.05)。克隆形成实验提示单纯照射组在2Gy时细胞存活分数(surviving fraction at 2Gy,SF2)为0.717±0.062;照射增敏组SF2为0.426±0.035,SER为1.684±0.178,差异有统计学意义(P=0.007)。FCM显示FADU细胞经4Gy照射后,G_2/M期细胞比例增加,G_1期细胞比例减少;经白藜芦醇作用24 h后,G_1期细胞比例减少,G_2/M期和S期细胞比例增加;当白藜芦醇与4 Gy照射联合应用时,G_2/M期细胞比例显著增加,G_1期细胞比例显著减少。对照组、单纯照射组、白藜芦醇组和照射增敏组FADU细胞的凋亡率(%)分别为1.94±1.65、4.56±0.92、2.03±1.46及23.1 1±7.22,照射增敏组凋亡率与其他各处理组比较,差异均有统计学意义(P<0.05)。结论白藜芦醇可增强咽鳞癌细胞株FADU放射敏感性,其机制可能与改变细胞周期的分布、诱导细胞凋亡相关。Objective To study the radiosensitizing effect of resveratrol on hypopharyngeal carcinoma cell line FADU in vitro.Methods Hypopharyngeal carcinoma cell line FADU was cultured in in vitro DMEM.Its inhibition on cell proliferation was detected using cytotoxicity test（MTT assay）.The cell survival curve was drawn using clone formation to obtain sensitive enhancement ratio（SER）.Changes of the cell cycle and cell apoptosis were analyzed using flow cytometry（FCM）.Results Results of MTT showed the inhibition of resveratrol on FADU cells increased along with its concentrations（P〈0.05）.Results of clone formation indicated the surviving fraction at 2 Gy（SF2） was 0.717 ±0.062 in the irradiation group,and 0.426 ±0.035 in the resveratrol plus irradiation group（with SER ranged 1.684 ±0.178）with statistical difference（P=0.007）.Results of FCM showed that after radiation of 4 Gy radiation,cells at G2/M phase arrest increased,but cells at G1 decreased.After radiation of resveratrol for 24 h,cells at G1 decreased,but cells at G2/M phase and S phase arrest increased.When 4 Gy radiation combined resveratrol was used,cells at G2/M phase arrest significantly increased,but cells at G1 significantly decreased.The apoptosis rate was 1.94%±1.65%in the control group,4.56%±0.92%in the irradiation group,2.03%±1.46%in the resveratrol group,and 23.11%±7.22%in the resveratrol plus irradiation group.There was statistical difference between the resveratrol plus irradiation group and the rest 3 groups（P〈0.05）.Conclusion Resveratrol could enhance the radiosensitivity of hypopharyngeal carcinoma FADU cells in vitro possibly by inducing cell apoptosis and causing changes in the cell cycle distribution.

关 键 词：白藜芦醇 放射增敏 凋亡 细胞周期 咽鳞癌 

分 类 号：R285[医药卫生—中药学]