补肾温阳化瘀方对实验性子宫内膜异位症TRPV1及其致敏因子NGF的影响  

Effects of Bushen Wenyang Huayu Recipe on TRPV1 and Sensitization Factor NGF in Experimental Endometriosis

作　　者：陈景伟[1] 仝瑞晓[2] 杨剑 李清雪[4] 马惠荣[1] 杜惠兰[1] 

机构地区：[1]河北中医学院中西医结合学院中西医结合生殖疾病协同创新中心,石家庄050200 [2]河北省邯郸市中医院妇科,河北056000 [3]河北省优抚医院内科,石家庄050051 [4]河北省石家庄市第四医院妇科,石家庄050011

出　　处：《中国中西医结合杂志》2015年第6期717-723,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：河北中医学院青年基金资助项目(No.QNZ2014002);河北中医学院博士科研基金项目(No.BSZ2015002)

摘　　要：目的研究补肾温阳化瘀方对实验性子宫内膜异位症(endometriosis,EMT)简称内异症,瞬时受体电位辣椒素亚型Ⅰ(transientreceptor potential vanilloid receptor 1,TRPV1)及致敏因子神经生长因子(nerve growth factor,NGF)的影响,以探讨补肾温阳化瘀方治疗EMT疼痛的作用机制。方法将近交系BALB/c雌性小鼠随机分为假手术组、模型组、中药高剂量组、中药低剂量组、孕三烯酮组,每组15只。观察各组扭体反应,并采用酶联免疫法(ELISA)检测血清NGF的含量,采用免疫组化SP法及Western blot法检测子宫和异位灶NGF、TRPV1蛋白的表达,采用Real-time PCR方法检测子宫和异位灶NGF、TRPV1 mRNA的表达。结果模型组血清NGF含量显著高于假手术组(P<0.01),且与小鼠扭体次数呈正相关(r=0.574,P<0.01);与模型组比较,中药高、低剂量组、孕三烯酮组血清NGF含量显著下降(P<0.05);模型组子宫NGF、TRPV1蛋白及NGF、TRPV1 mRNA表达均高于假手术组(P<0.01)。与模型组比较,中药高、低剂量组、孕三烯酮组子宫和异位灶NGF、TRPV1蛋白表达均显著降低(P<0.01),NGF、TRPV1 mRNA以中药高剂量组下降最为明显(P<0.01)。子宫及异位灶NGF与TRPV1蛋白及mRNA表达均呈正相关(P<0.01)。结论 TRPV1及致敏因子NGF共同参与了EMT疼痛的发生;补肾温阳化瘀方可通过降低NGF对TRPV1的上调作用从而发挥抑制EMT疼痛的作用。Objective To study the effect of Bushen Wenyang Huayu Recipe（BSWYHYR） on nerve growth factor（NGF） and transient receptor potential vanilloid receptor Ⅰ（TRPV1） in experimental endometriosis（EMT）,and to explore its mechanism for treating EMT-induced pain.Methods Totally inbred line BALB/c 75 female mice were divided into five groups,i.e.,the sham-operation group,the model group,the high dose BSWYHYR group,the low dose BSWYHYR group,the gestrinone group,15 in each group.Writhing response was observed in each group.Serum contents of NGF were detected using ELISA.Expression levels of NGF and TRPV1 in uterus and ectopic foci were detected using immunohistochemical staining SP and Western blot.mRNA expression levels of NGF and TRPV1 in uterus and ectopic foci were detected by Real-time PCR.Results The serum NGF content in the model group was higher than that in the sham-operation group（P 〈0.01）,and there was positive correlation between NGF and the writhing frequency（r =0.574,P 〈0.01）.Compared with the model group,serum levels of NGF significantly decreased in the 3 treatment groups（P〈0.05）.Compared with the sham-operation group,mRNA and protein expression levels of NGF and TRPV1 increased significantly in the model group（P 〈0.01）.Protein expression levels of NGF and TRPV1 decreased significantly in the 3 treatment groups,when compared with the model group（P 〈0.01）.mRNA expression levels of NGF and TRPV1 decreased most in the high dose BSWYHYR group（P〈0.01）.NGF in uterus and ectopic foci was positively correlated with protein and mRNA expression levels of TRPV1（P 〈0.01）.Conclusions NGF and TRPV1 participated in the occurrence of pain in EMS.BSWYHYR played an important role in inhibiting EMT-induced pain through reducing the up-regulation of NGF on TRPV1.

关 键 词：子宫内膜异位症 神经生长因子 瞬时受体电位辣椒素亚型Ⅰ 补肾温阳化瘀方 疼痛 

分 类 号：R285.5[医药卫生—中药学]