尿道下裂患儿包皮组织中E盒结合锌指蛋白1的表达  

Expression of ZEB1 in the prepuce of hypospadias children and its implication

作　　者：周阳[1] 乔梁[2] 李永响[2] 张居民[3] 刘鲁东[2] 卢洪凯[2] 

机构地区：[1]潍坊医学院外科教研室,山东潍坊261042 [2]潍坊医学院附属潍坊市人民医院泌尿外科,山东潍坊261042 [3]潍坊医学院附属潍坊市人民医院病理科,山东潍坊261042

出　　处：《中华男科学杂志》2015年第6期510-514,共5页National Journal of Andrology

基　　金：山东省医药卫生科技发展计划项目(2011QW001)~~

摘　　要：目的:探讨E盒结合锌指蛋白1(ZEB1)在尿道下裂患者包皮组织中的表达,分析ZEB1 mRNA和蛋白的表达水平与尿道下裂的关系。方法:实验组和对照组的包皮组织来自于37例尿道下裂修复术患儿和11例包皮环切术包皮过长患儿,37例尿道下裂根据尿道口的位置归类为24例轻中度尿道下裂和13例重度尿道下裂,通过免疫组化和RT-PCR法分别对ZEB1的蛋白和mRNA表达水平进行检测。结果:ZEB1蛋白阳性表达率在轻中度尿道下裂组为75.0%(18/24)、重度尿道下裂组为100%(13/13),明显高于对照组的9.1%(1/11),且两两比较均具有统计学意义(P均<0.05);RT-PCR显示ZEB1 mRNA表达在对照组,轻中度、重度尿道下裂组织中的相对累计光密度值比分别为(0.67±0.21)、(0.81±0.24)、(1.55±0.29),除对照组和轻中度尿道下裂组差异无统计学意义外(P=0.64),对照组和轻中度尿道下裂组分别和重度尿道下裂组比较差异均有统计学意义(P均<0.05)。结论:ZEB1在尿道下裂患儿的包皮组织中的表达明显上调,且与尿道下裂的严重程度相关,提示ZEB1基因表达上调可能导致尿道下裂的形成。Objective： To investigate the expression of zinc finger E-box binding homebox 1（ZEB1） in the prepuce of hypospadias children and its relationship to the incidence of hypospadias. Methods： Prepuce tissues were collected from 37 children aged6- 15 months undergoing hypospadias repair and 11 age-matched controls receiving circumcision. Based on the position of the urethral meatus,the hypospadias cases were classified as severe（ n = 13） and mild-moderate（ n = 24）. The mRNA and protein expressions of ZEB1 were determined by immunohistochemistry and RT-PCR. Results： The expression of the ZEB1 protein was remarkably higher in the severe（100% [13 /13]） and mild-moderate hypospadias patients（75. 0% [18 /24]） than in the controls（9. 1% [1 /11]）,with statistically significant differences between any two groups（ P 〈 0. 05）. RT-PCR showed the integrated density value（ IDV） of the ZEB1 mRNA expression to be（0. 67 ± 0. 21）,（0. 81 ± 0. 24）,and（1. 55 ± 0. 29） in the control,mild-moderate,and severe hypospadias patients,respectively,significantly higher in the severe hypospadias than in the control and mild-moderate hypospadias groups（P 〈 0. 05）,but with no significant difference between the latter two（P = 0. 64）. Conclusion： The expression of ZEB1 is significantly increased in hypospadias patients,and its upregulation is positively correlated with the severity of hypospadias,which suggests that the overexpression of ZEB1 may contribute to the development of hypospadias.

关 键 词：尿道下裂 E盒结合锌指蛋白1 上皮间质转化 

分 类 号：R726.9[医药卫生—儿科]