血管紧张素(Ang)Ⅱ、Ang-(1-7)对人卵巢黄素化颗粒细胞的增殖及分泌功能的影响  

Effects of angiotensin(Ang)Ⅱ, Ang-(1-7) on the proliferation and secretory function of human luteinized granulosa cells

作　　者：陈丽华[1] 刘洁[2] 李晓冬[2] 

机构地区：[1]河北北方学院附属第一医院,张家口075000 [2]河北医科大学第二医院,石家庄050000

出　　处：《生殖与避孕》2015年第6期366-372,共7页Reproduction and Contraception

摘　　要：目的：了解血管紧张素（Ang）Ⅱ、Ang-（1-7）对人卵巢黄素化颗粒细胞的增殖及分泌功能的影响。方法：提取行体外受精/卵胞质内单精子注射-胚胎移植（IVF/ICSI-ET）患者卵泡液中的颗粒细胞,经过提纯后随机分为实验组与对照组。实验组根据添加的药物分为AngⅡ组与Ang-（1-7）组。分别向AngⅡ组/Ang-（1-7）组的颗粒细胞培养液中加入10-10~10-5 mmol/L浓度的AngⅡ/Ang-（1-7）,对照组的颗粒细胞培养液中不添加药物,各组分别作用24 h、48 h、72 h,用四甲基偶氮唑蓝盐比色法（MTT法）测定颗粒细胞的吸光度（D）值;向AngⅡ组/Ang-（1-7）组的颗粒细胞培养液中分别加入10-6 mmol/L浓度的AngⅡ/Ang-（1-7）,对照组的培养液中不添加药物,收集培养0~24 h、24~48 h、48~72 h的上清液,化学发光分析法（CL）测定黄素化颗粒细胞上清液中雌二醇（E2）、孕酮（P）水平。结果：不同浓度的AngⅡ、Ang-（1-7）分别作用颗粒细胞后,其D值均较对照组低,并随着浓度增加D值逐渐降低;随着时间延长,各组D值逐渐升高。10-6 mmol/L浓度的AngⅡ、Ang-（1-7）分别作用于颗粒细胞后,其上清液中E2的浓度均较对照组高,P浓度与对照组无统计学差异,随着时间延长,各组的E2和P含量逐渐增加。结论：AngⅡ及Ang-（1-7）对人卵巢黄素化颗粒细胞生长起抑制作用,其抑制作用随浓度增加逐渐增强。AngⅡ、Ang-（1-7）能够增强人卵巢黄素化颗粒细胞分泌雌激素的作用,但对孕激素的分泌没有影响。提示AngⅡ、Ang-（1-7）在人卵巢黄素化颗粒细胞的增殖及激素分泌中起一定作用,两者可能参与了人类的卵泡闭锁、优势卵泡发育、成熟以及排卵等生理过程。Objective： To investigate the effects of angiotensin（Ang）Ⅱ, Ang-（1-7） on the proliferation and secretory function of human luteinized granulosa cells. Methods： The granulosa cells in the follicular fluid were extracted from the patients undergoing IVF/ICSI-ET cycles, and were randomly divided into experimental group and control group after purification. According to adding different drugs, the experimental group was divided into Ang IⅡ group and Ang-（1-7） group. Granulosa cells of AngⅡ group were interfered with the AngⅡ of 10-10-10-5 mmol/L dose, granulosa cells of Ang-（1-7） group were interfered with the Ang-（1-7） of10-10-10-5 mmol/L dose. The control was interfered without drug. All of the groups were cultured by 24 h, 48 h and72 h, respectively, D values were determined by MTT method; AngⅡ group and Ang-（1-7） group were interfered with AngⅡ and Ang-（1-7） of 10-6 mmol/L dose respectively, the control was interfered without drug. All of the groups were cultured and the supernatant was collected by 24 h, 48 h and 72 h. The estradiol（E2） and progesterone（P） levels in the culture medium were detected by chemiluminescence method. Results： Compared with control group, all the D values of granulosa cells interfered with AngⅡ, Ang-（1-7） in different doses and at different time were lower（P〈0.05）, and the D values gradually reduced with the increase of concentrations, the D values of all groups were increased as time went by. Compared with control group, the E2 levels of AngⅡ group and Ang-（1-7） group were higher which interfered with AngⅡ and Ang-（1-7） of 10-6 mmol/L, respectively at the different time, but the P levels of AngⅡ group, Ang-（1-7） group and control group had no obvious difference. As time went by, the E2,P levels of all the groups were increased. Conclusion： Both of AngⅡ and Ang-（1-7） restrained the growth of the granulosa cells, and the effect was enhanced with increased dose. Both AngⅡ and Ang-（1-7） promot

关 键 词：血管紧张素(Ang)Ⅱ 血管紧张素1-7[Ang-(1-7)] 人卵巢黄素化颗粒细胞 细胞增殖 雌二醇(E2) 孕酮(P) 

分 类 号：R714.8[医药卫生—妇产科学]